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Degradation Properties of a Bi-layered Cross-linked Collagen Membrane for Localized Bone Regeneration: In Vitro and In Vivo Study

 Jin-Young Park  ;  Jae-Hong Lee  ;  Jae-Kook Cha  ;  Jung-Seok Lee  ;  Ui-Won Jung  ;  Seong-Ho Cho 
 Journal of Korean Dental Science, Vol.14(1) : 12-25, 2021-06 
Journal Title
Journal of Korean Dental Science
Issue Date
Alveolar bone grafting ; Biocompatible materials ; Bone regeneration ; Collagen
Purpose: (i) To evaluate the biologic properties of a bi-layered 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride-cross-linked collagen membrane (CCM) in vitro. (ii) To assess the efficacy of CCM for localized bone regeneration in vivo. Materials and Methods: Biodegradation of CCM compared to a native collagen membrane (NCM) was assessed in vitro. In vivo, twelve male New Zealand White rabbits were used. Four calvarial, circular defects (diameter 8 mm) were created in each animal. The sites were randomly allocated to i) CCM+biphasic calcium phosphate (BCP) (CCM-BCP group), ii) CCM alone (CCM), iii) BCP alone (BCP) and, iv) negative control (control). Animals were sacrificed at 2 (n=6) and 8 weeks (n=6). Outcome measures included: micro-computed tomography (μCT) analysis (total augmented volume [TAV], new bone volume) and histomorphometry (total augmented area [TAA], newly formed bone, remaining membrane thickness [RMT]). Result: CCM was more resistant to degradation than NCM. μCT analysis showed CCM-BCP (196.43±25.30 mm3) and BCP (206.23±39.13 mm3) groups had significantly (P<0.01) larger TAV than the control (149.72±12.28 mm3) after 8 weeks. Histomorphometrically, CCM-BCP group (17.75±5.97 mm2) had significantly (P<0.01) greater TAA compared to the CCM group (7.74±2.25 mm2) and the control (8.13±1.81 mm2) after 8 weeks. After 8 weeks, RMT was reduced by 67%. Conclusion: CCM can be a favorable choice of barrier membrane when performing guided bone regeneration (GBR) in localized bone defects. CCM has better resistance to degradation than the natural collagen membrane, in vitro. In vivo, CCM provides an advantageous integration of prolonged barrier function and biocompatibility for GBR.
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2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Park, Jin Young(박진영)
Lee, Jung Seok(이중석) ORCID logo https://orcid.org/0000-0003-1276-5978
Jung, Ui Won(정의원) ORCID logo https://orcid.org/0000-0001-6371-4172
Cha, Jae Kook(차재국) ORCID logo https://orcid.org/0000-0001-6817-9834
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
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