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Isolation and pure culture of microvascular endothelial cells from the fetal skin

Authors
 Myung Soo Cha  ;  Dong Kyun Rah  ;  Kwang Hoon Lee 
Citation
 YONSEI MEDICAL JOURNAL, Vol.37(3) : 186-193, 1996-06 
Journal Title
YONSEI MEDICAL JOURNAL
ISSN
 0513-5796 
Issue Date
1996-06
MeSH
Cells, Cultured ; Endothelium, Vascular / cytology* ; Factor VIII / analysis ; Female ; Fetus ; Humans ; Intercellular Adhesion Molecule-1 / analysis ; Pregnancy ; Skin / blood supply ; Vascular Cell Adhesion Molecule-1 / analysis
Abstract
Microvascular endothelial cells were purely isolated from human fetal skin using magnetic particles. The principle of this technique is based on the selective binding of the lectin Ulex europaeus I (UEA I) to the endothelial cell surface via fucose residues. Initially UEA I was covalently bound to tosyl-activated magnetic polydisperse polymer particles (Dynabeads) and then the UEA I-coated beads were collected using a magnetic particle concentrator (MPC). Endothelial cells were isolated by extracting microvascular segments from trypsin-treated fetal skin tissue and were purified by sieving with nylon mesh and by 35% Percoll gradient centrifugation. For further purification, the obtained cells were incubated with UEA I-coated Dynabeads. The endothelial cells bound to the Dynabeads were collected using MPC. This is a simple and reproducible technique for isolating a pure population of microvascular endothelium from the fetal skin.
Files in This Item:
T199602112.pdf Download
DOI
10.3349/ymj.1996.37.3.186
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Rah, Dong Kyun(나동균)
Lee, Kwang Hoon(이광훈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/183568
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