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Isolation and pure culture of microvascular endothelial cells from the fetal skin
DC Field | Value | Language |
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dc.contributor.author | 나동균 | - |
dc.contributor.author | 이광훈 | - |
dc.date.accessioned | 2021-09-28T08:18:48Z | - |
dc.date.available | 2021-09-28T08:18:48Z | - |
dc.date.issued | 1996-06 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/183568 | - |
dc.description.abstract | Microvascular endothelial cells were purely isolated from human fetal skin using magnetic particles. The principle of this technique is based on the selective binding of the lectin Ulex europaeus I (UEA I) to the endothelial cell surface via fucose residues. Initially UEA I was covalently bound to tosyl-activated magnetic polydisperse polymer particles (Dynabeads) and then the UEA I-coated beads were collected using a magnetic particle concentrator (MPC). Endothelial cells were isolated by extracting microvascular segments from trypsin-treated fetal skin tissue and were purified by sieving with nylon mesh and by 35% Percoll gradient centrifugation. For further purification, the obtained cells were incubated with UEA I-coated Dynabeads. The endothelial cells bound to the Dynabeads were collected using MPC. This is a simple and reproducible technique for isolating a pure population of microvascular endothelium from the fetal skin. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Endothelium, Vascular / cytology* | - |
dc.subject.MESH | Factor VIII / analysis | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Fetus | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Intercellular Adhesion Molecule-1 / analysis | - |
dc.subject.MESH | Pregnancy | - |
dc.subject.MESH | Skin / blood supply | - |
dc.subject.MESH | Vascular Cell Adhesion Molecule-1 / analysis | - |
dc.title | Isolation and pure culture of microvascular endothelial cells from the fetal skin | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Plastic and Reconstructive Surgery (성형외과학교실) | - |
dc.contributor.googleauthor | Myung Soo Cha | - |
dc.contributor.googleauthor | Dong Kyun Rah | - |
dc.contributor.googleauthor | Kwang Hoon Lee | - |
dc.identifier.doi | 10.3349/ymj.1996.37.3.186 | - |
dc.contributor.localId | A01229 | - |
dc.contributor.localId | A02674 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 8826784 | - |
dc.contributor.alternativeName | Rah, Dong Kyun | - |
dc.contributor.affiliatedAuthor | 나동균 | - |
dc.contributor.affiliatedAuthor | 이광훈 | - |
dc.citation.volume | 37 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 186 | - |
dc.citation.endPage | 193 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.37(3) : 186-193, 1996-06 | - |
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