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Developmental Roles of FUSE Binding Protein 1 ( Fubp1) in Tooth Morphogenesis

Authors
 Yam Prasad Aryal  ;  Sanjiv Neupane  ;  Tae-Young Kim  ;  Eui-Seon Lee  ;  Nitin Kumar Pokhrel  ;  Chang-Yeol Yeon  ;  Ji-Youn Kim  ;  Chang-Hyeon An  ;  Seo-Young An  ;  Eui-Kyun Park  ;  Jung-Hong Ha  ;  Jae-Kwang Jung  ;  Hitoshi Yamamoto  ;  Sung-Won Cho  ;  Sanggyu Lee  ;  Do-Yeon Kim  ;  Tae-Yub Kwon  ;  Youngkyun Lee  ;  Wern-Joo Sohn  ;  Jae-Young Kim 
Citation
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, Vol.21(21) : 8079, 2020-10 
Journal Title
 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 
Issue Date
2020-10
MeSH
Animals ; Cell Proliferation ; DNA-Binding Proteins / genetics ; DNA-Binding Proteins / metabolism* ; Embryo, Mammalian / cytology* ; Embryo, Mammalian / metabolism ; Gene Expression Regulation, Developmental* ; Mice ; Mice, Inbred ICR ; Morphogenesis* ; Odontogenesis* ; RNA-Binding Proteins / genetics ; RNA-Binding Proteins / metabolism* ; Signal Transduction ; Tooth / embryology* ; Tooth / metabolism
Keywords
Fubp1 ; amelogenesis ; dentinogenesis ; tooth development ; transcriptional regulator
Abstract
FUSE binding protein 1 (Fubp1), a regulator of the c-Myc transcription factor and a DNA/RNA-binding protein, plays important roles in the regulation of gene transcription and cellular physiology. In this study, to reveal the precise developmental function of Fubp1, we examined the detailed expression pattern and developmental function of Fubp1 during tooth morphogenesis by RT-qPCR, in situ hybridization, and knock-down study using in vitro organ cultivation methods. In embryogenesis, Fubp1 is obviously expressed in the enamel organ and condensed mesenchyme, known to be important for proper tooth formation. Knocking down Fubp1 at E14 for two days, showed the altered expression patterns of tooth development related signalling molecules, including Bmps and Fgf4. In addition, transient knock-down of Fubp1 at E14 revealed changes in the localization patterns of c-Myc and cell proliferation in epithelium and mesenchyme, related with altered tooth morphogenesis. These results also showed the decreased amelogenin and dentin sialophosphoprotein expressions and disrupted enamel rod and interrod formation in one- and three-week renal transplanted teeth respectively. Thus, our results suggested that Fubp1 plays a modulating role during dentinogenesis and amelogenesis by regulating the expression pattern of signalling molecules to achieve the proper structural formation of hard tissue matrices and crown morphogenesis in mice molar development.
Files in This Item:
T202006929.pdf Download
DOI
10.3390/ijms21218079
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/182804
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