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Comparison of Biocompatibility of Calcium Silicate-Based Sealers and Epoxy Resin-Based Sealer on Human Periodontal Ligament Stem Cells

 Hanseul Oh  ;  Egan Kim  ;  Sukjoon Lee  ;  Soyeon Park  ;  Dongzi Chen  ;  Su-Jung Shin  ;  Euiseong Kim  ;  Sunil Kim 
 MATERIALS, Vol.13(22) : 5242, 2020-11 
Journal Title
Issue Date
biocompatibility ; calcium silicate-based sealer ; cell viability ; epoxy resin-based sealer ; inflammatory response ; periodontal ligament stem cell ; root canal treatment
The aim of this study was to evaluate the biocompatibility of calcium silicate-based sealers (CeraSeal and EndoSeal TCS) and epoxy resin-based sealer (AH-Plus) in terms of cell viability, inflammatory response, expression of mesenchymal phenotype, osteogenic potential, cell attachment, and morphology, of human periodontal ligament stem cells (hPDLSCs). hPDLSCs were acquired from the premolars (n = 4) of four subjects, whose ages extended from 16 to 24 years of age. Flow cytometry analysis showed stemness of hPDLSCs was maintained in all materials. In cell viability test, AH-Plus showed the lowest cell viability, and CeraSeal showed significantly higher cell viability than others. In ELISA test, AH-Plus showed higher expression of IL-6 and IL-8 than calcium silicate-based sealers. In an osteogenic potential test, AH-Plus showed a lower expression level than other material; however, EndoSeal TCS showed a better expression level than others. All experiments were repeated at least three times per cell line. Scanning electronic microscopy studies showed low degree of cell proliferation on AH-Plus, and high degree of cell proliferation on calcium silicate-based sealers. In this study, calcium silicate-based sealers appear to be more biocompatible and less cytotoxic than epoxy-resin based sealers.
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sun Il(김선일) ORCID logo https://orcid.org/0000-0002-8889-9844
Kim, Eui Seong(김의성) ORCID logo https://orcid.org/0000-0003-2126-4761
Shin, Su Jung(신수정) ORCID logo https://orcid.org/0000-0001-5795-3919
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