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Cell Surface Antigen Display for Neuronal Differentiation-Specific Tracking

Authors
 Sang Chul Kim  ;  Eun-Hye Lee  ;  Ji Hea Yu  ;  Sang-Mi Kim  ;  Bae-Geun Nam  ;  Hee Yong Chung  ;  Yeon-Soo Kim  ;  Sung-Rae Cho  ;  Chang-Hwan Park 
Citation
 BIOMOLECULES & THERAPEUTICS, Vol.27(1) : 78-84, 2019-01 
Journal Title
 BIOMOLECULES & THERAPEUTICS 
ISSN
 1976-9148 
Issue Date
2019-01
Keywords
Differentiation ; In vivo monitoring ; Neuron specific ; Surface antigen
Abstract
Cell therapeutic agents for treating degenerative brain diseases using neural stem cells are actively being developed. However, few systems have been developed to monitor in real time whether the transplanted neural stem cells are actually differentiated into neurons. Therefore, it is necessary to develop a technology capable of specifically monitoring neuronal differentiation in vivo. In this study, we established a system that expresses cell membrane-targeting red fluorescent protein under control of the Synapsin promoter in order to specifically monitor differentiation from neural stem cells into neurons. In order to overcome the weak expression level of the tissue-specific promoter system, the partial 5' UTR sequence of Creb was added for efficient expression of the cell surface-specific antigen. This system was able to track functional neuronal differentiation of neural stem cells transplanted in vivo, which will help improve stem cell therapies.
Files in This Item:
T201900139.pdf Download
DOI
10.4062/biomolther.2018.110
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Rehabilitation Medicine (재활의학교실) > 1. Journal Papers
Yonsei Authors
Cho, Sung-Rae(조성래) ORCID logo https://orcid.org/0000-0003-1429-2684
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/179870
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