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3D Imaging of the Transparent Mycobacterium tuberculosis-Infected Lung Verifies the Localization of Innate Immune Cells With Granuloma

Authors
 Gyeong-Yi Kang  ;  Hyeong-Jun Rhyu  ;  Hong-Hee Choi  ;  Sung Jae Shin  ;  Young-Min Hyun 
Citation
 FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, Vol.10 : 226, 2020-05 
Journal Title
 FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 
Issue Date
2020-05
Keywords
granuloma ; innate immunity ; lung ; tissue clearing ; tuberculosis
Abstract
Using a novel tissue-clearing method, we aimed to visualize the three-dimensional (3D) distribution of immune cells within Mycobacterium tuberculosis (Mtb)-infected mice lungs. Ethyl cinnamate-based tissue clearing of Mtb-infected mice lungs was performed to obtain transparent lung samples, which were then imaged using a light sheet fluorescence microscope. Using the 3D images, we performed quantitative analysis of the immune cell population within multiple granulomas. In addition, to compare the data from the tissue clearing method, we performed histopathological and immunofluorescence analyses, and flow cytometry. We then created 3D images of the Mtb-infected lung that successfully demonstrated the distribution of blood vessels, immune cells, and granulomas. Since the immune cells within a granuloma could be separately selected and counted, the immune cell population within a specific lesion could be quantified. In addition, macroscopic analysis, e.g., the size or shape of a granuloma, as well as microscopic analysis could be performed as intact lung samples were used. The use of the tissue clearing method in infected lungs could be a novel modality for understanding the role of the immune system in the pathogenesis of tuberculosis.
Files in This Item:
T202002147.pdf Download
DOI
10.3389/fcimb.2020.00226
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Sung Jae(신성재) ORCID logo https://orcid.org/0000-0003-0854-4582
Hyun, Young-Min(현영민) ORCID logo https://orcid.org/0000-0002-0567-2039
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/179214
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