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Serpina3c Regulates Adipogenesis by Modulating Insulin Growth Factor 1 and Integrin Signaling

Authors
 Yoonjeong Choi  ;  Hyeonjin Choi  ;  Bo Kyung Yoon  ;  Hyemin Lee  ;  Jo Woon Seok  ;  Hyo Jung Kim  ;  Jae-Woo Kim 
Citation
 ISCIENCE, Vol.23(3) : 100961, 2020-03 
Journal Title
 ISCIENCE 
Issue Date
2020-03
Keywords
Developmental Biology ; Molecular Biology ; Transcriptomics
Abstract
Preadipocyte differentiation can be induced upon a hormonal treatment, and various factors secreted by the cells may contribute to adipogenesis. In this study, RNA-seq revealed Serpina3c as a critical factor regulating the signaling network during adipogenesis. Serpina3c is a secretory protein and is highly expressed in fat tissues. Knockdown of Serpina3c decreased adipogenesis by attenuating the mitotic clonal expansion of 3T3-L1 cells. These cells exhibited decreases in integrin α5, which abolished the phosphorylation of integrin β3. We found that Serpina3c inhibits a serine protease that regulates integrin α5 degradation. Knockdown of Serpina3c disrupted integrin-mediated insulin growth factor 1 (IGF-1) signaling and ERK activation. Serpina3c-mediated regulation of integrin-IGF-1 signaling is also associated with AKT activation, which affects the nuclear translocation of GSK3β. Altogether, our results indicate that Serpina3c secreted from differentiating adipocytes inhibits serine proteases to modulate integrin/IGF-1-mediated ERK and AKT signaling and thus is a critical factor contributing to adipogenesis.
Files in This Item:
T202002255.pdf Download
DOI
10.1016/j.isci.2020.100961
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
3. College of Nursing (간호대학) > Dept. of Nursing (간호학과) > 1. Journal Papers
Yonsei Authors
Kim, Jae Woo(김재우) ORCID logo https://orcid.org/0000-0001-5456-9495
Seok, Jo Woon(석조운)
Yoon, Bo Kyung(윤보경)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/179025
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