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IFN-γ Regulates Expression of BRG1 Associated Factor 155/170 and Sensitivity to Steroid in Astrocytes

Authors
 Jung-Hee Lim1, Jeonggi Lee1, Joo Young Park2 and In-Hong Choi1 
Citation
 IMMUNE NETWORK, Vol.4(4) : 224-228, 2004-12 
Journal Title
 IMMUNE NETWORK 
ISSN
 1598-2629 
Issue Date
2004-12
Keywords
Astrocyte ; steroid ; BAF155/170 ; IFN-γ
Abstract
Background: The expression of BRG1 associated factors (BAF) 155 and BAF 170 in response to IFN-γ or TNF-α was studied in astrocytoma cell lines and primary astrocytes. BAFs are complexed with BRG1 and are also associated with activated glucocorticoid for glucocorticoid trans-activation. Methods: IFN-γ was pretreated for 18 hrs and cells were incubated with IL-1 or TNF-α for 72 hrs or 96 hrs with different concentrations of steroid. Cell death was measured by LDH assay. BAF expression was assayed by RT-PCR. Results: IFN-γ increased cell death by dexamethasone in LN215 cells but not in LN319 cells. The IFN-γ increased the expression of BAF 155 and BAF 170 in adult astrocytes and LN215 cells, but IFN-γ decreased the expression of BAF 155/170 in LN319 cells. The effect of IFN-γ on the expression of BAF was not as clear in fetal astrocytes as it was in adult astrocytes. Conclusion: Our results suggest cytokines produced during immune reaction or immunotherapy may modulate steroid susceptibility of astrocytes and astrocytoma cells by influencing the expression of BAFs.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/178791
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