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IFN-γ Regulates Expression of BRG1 Associated Factor 155/170 and Sensitivity to Steroid in Astrocytes

 Jung-Hee Lim1, Jeonggi Lee1, Joo Young Park2 and In-Hong Choi1 
 IMMUNE NETWORK, Vol.4(4) : 224-228, 2004-12 
Journal Title
Issue Date
Astrocyte ; steroid ; BAF155/170 ; IFN-γ
Background: The expression of BRG1 associated factors (BAF) 155 and BAF 170 in
response to IFN-γ or TNF-α was studied in astrocytoma cell lines and primary
astrocytes. BAFs are complexed with BRG1 and are also associated with activated
glucocorticoid for glucocorticoid trans-activation. Methods: IFN-γ was pretreated for
18 hrs and cells were incubated with IL-1 or TNF-α for 72 hrs or 96 hrs with different
concentrations of steroid. Cell death was measured by LDH assay. BAF expression was
assayed by RT-PCR. Results: IFN-γ increased cell death by dexamethasone in LN215
cells but not in LN319 cells. The IFN-γ increased the expression of BAF 155 and
BAF 170 in adult astrocytes and LN215 cells, but IFN-γ decreased the expression of
BAF 155/170 in LN319 cells. The effect of IFN-γ on the expression of BAF was
not as clear in fetal astrocytes as it was in adult astrocytes. Conclusion: Our results
suggest cytokines produced during immune reaction or immunotherapy may modulate
steroid susceptibility of astrocytes and astrocytoma cells by influencing the expression
of BAFs.
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1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
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