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Molecular basis of HLA-C recognition by p58 natural killer cell inhibitory receptors

 Jongsun Kim  ;  Yong Joon Chwae  ;  Mi Yeon Kim  ;  In Hong Choi  ;  Jeon Han Park  ;  Se Jong Kim 
 JOURNAL OF IMMUNOLOGY, Vol.159(8) : 3857-3882, 1997 
Journal Title
Issue Date
Amino Acid Sequence ; Antigens/physiology ; Blood Donors ; Cloning, Molecular ; DNA, Complementary/isolation & purification ; HLA-C Antigens/chemistry ; HLA-C Antigens/genetics ; HLA-C Antigens/metabolism* ; Humans ; Immunoglobulins/physiology ; Killer Cells, Natural/metabolism* ; Molecular Sequence Data ; Peptides/immunology ; Peptides/physiology ; Protein Binding ; Protein Folding ; Protein Structure, Tertiary ; Receptors, Immunologic/biosynthesis ; Receptors, Immunologic/chemistry* ; Receptors, Immunologic/genetics ; Receptors, KIR ; Receptors, KIR2DL3 ; Receptors, Natural Killer Cell ; Solubility
NK cells express several inhibitory receptors that recognize class I MHC molecules expressed on target cells. The NK cell inhibitory receptors (KIRs) provide a key regulatory function for NK cells via specific interaction with MHC/peptide complexes, but the molecular details for recognition of class I MHC molecules by KIRs still remain unclear. Here we report cDNA cloning and expression of p58 KIRs and a p50 killer cell activatory receptor (KAR) from a Korean blood donor and demonstrate direct binding between recombinant soluble p58 KIRs and recombinant soluble HLA-C molecules. We identified three p58/p50 killer cell receptors (KAR-K1, KIR-K6, and KIR-K7), which are homologous to p50 cl-39, p58 47.11, and p58 cl-6, respectively. Native gel shift assay revealed that p58 KIR-K6 and KIR-K7 bind both HLA-Cw3 and HLA-Cw6 molecules, but p50 KAR-K1 binds neither of the HLA-C molecules. However, binding of HLA-C molecule by p58 KIR is affected by the antigenic peptide bound on the MHC molecule, suggesting that the p58 KIR binding to the HLA-C molecule may be dependent on the peptide. In addition, the binding interaction requires the presence of both p58 Ig domains, suggesting that the binding mode of HLA-C and p58 KIR may have some similarity to that of the neonatal Fc receptor and the Fc fragment of Ab and may be distinct from that of TCR and MHC.
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1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Jong(김세종)
Kim, Jong Sun(김종선) ORCID logo https://orcid.org/0000-0002-3149-669X
Park, Jeon Han(박전한) ORCID logo https://orcid.org/0000-0001-9604-3205
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
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