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급속진행성치주염 환자로부터 배양분리한 Porphyromonas gingivalis균주의 ribotyping

Other Titles
 Ribotyping of Porphyromonas Gingivalis Isolated from Rapidly Progressive Periodontitis Patients 
Authors
 김진홍  ;  최봉규  ;  최성호  ;  조규성  ;  채중규  ;  김종관 
Citation
 Journal of Korean Academy of Periodontology (대한치주과학회지), Vol.29(4) : 963-977, 1999 
Journal Title
 Journal of Korean Academy of Periodontology (대한치주과학회지) 
ISSN
 0250-3352 
Issue Date
1999
Keywords
급속진행성치주염 ; PORPHYROMONAS GINGIVALIS ; RIBOTYPING
Abstract
This study examined ribotypes of 36 P. gingivalis strains isolated from 10 rapidly progressive periodontitis patients in Korean and revealed the presence of genetic heterogeneity among the patients. Ribotyping was performed by using a oligonucleotide probes based on 16S rRNA after whole genomic DNA had been digested with the restriction endonuclease enzyme Kpn I and Pst I. In addition, the antigenic heterogeneity of fimbrillin and protease activity was analysed to observe the virulency of P. gingivalis. The results were as follows. 1. Using KpnI, 6 ribotypes were detected, whereas 7 ribotypes were identified by using PstI. When combined two enzymes, a total of 8 ribotypes was subgrouped. 2. Ribotype I/e was the most common and detected in 4 among 10 patients. 3. The fimbrillin expressed from P. gingivalis isolates had the molecular size of 41kDa, 43kDa, 49kDa. It was observed that the size of fimbrillin with the same ribotypes could be identical. 4. All the P. gingivalis strains showed strong proteolytic activity and had the molecular size more than 120kDa. In summary, total 8 ribotypes were observed for isolates from rapidly progressive periodontitis patients. Forty percent of the patients harbored isolates exhibiting the same ribotype I/e, and it was observed that more than one ribotype can coexist in an individual patient.
Files in This Item:
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Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chong Kwan(김종관)
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
Chai, Jung Kyu(채중규)
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174366
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