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Activator-specific requirement of yeast mediator proteins for RNA polymerase II transcriptional activation.

Authors
 SANG JUN HAN  ;  YOUNG CHUL LEE  ;  BYUNG SOO GIM  ;  GI-HYUCK RYU  ;  SOON JUNG PARK  ;  WILLIAM S. LANE  ;  YOUNG-JOON KIM 
Citation
 Molecular and Cellular Biology, Vol.19(2) : 978-988, 1999 
Journal Title
 Molecular and Cellular Biology 
ISSN
 0270-7306 
Issue Date
1999
MeSH
Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; DNA Polymerase II/genetics* ; DNA Primers/genetics ; Fungal Proteins/chemistry ; Fungal Proteins/genetics ; Fungal Proteins/metabolism* ; Genes, Fungal ; Holoenzymes/genetics ; Humans ; Macromolecular Substances ; Molecular Sequence Data ; Mutation ; Protein Conformation ; Saccharomyces cerevisiae/enzymology ; Saccharomyces cerevisiae/genetics* ; Saccharomyces cerevisiae/metabolism* ; Sequence Homology, Amino Acid ; Transcriptional Activation
Abstract
The multisubunit Mediator complex of Saccharomyces cerevisiae is required for most RNA polymerase II (Pol II) transcription. The Mediator complex is composed of two subcomplexes, the Rgr1 and Srb4 subcomplexes, which appear to function in the reception of activator signals and the subsequent modulation of Pol II activity, respectively. In order to determine the precise composition of the Mediator complex and to explore the specific role of each Mediator protein, our goal was to identify all of the Mediator components. To this end, we cloned three previously unidentified Mediator subunits, Med9/Cse2, Med10/Nut2, and Med11, and isolated mutant forms of each of them to analyze their transcriptional defects. Differential display and Northern analyses of mRNAs from wild-type and Mediator mutant cells demonstrated an activator-specific requirement for each Mediator subunit. Med9/Cse2 and Med10/Nut2 were required, respectively, for Bas1/Bas2- and Gcn4-mediated transcription of amino acid biosynthetic genes. Gal11 was required for Gal4- and Rap1-mediated transcriptional activation. Med11 was also required specifically for MFalpha1 transcription. On the other hand, Med6 was required for all of these transcriptional activation processes. These results suggest that distinct Mediator proteins in the Rgr1 subcomplex are required for activator-specific transcriptional activation and that the activation signals mediated by these Mediator proteins converge on Med6 (or the Srb4 subcomplex) to modulate Pol II activity.
Files in This Item:
T199903211.pdf Download
DOI
10.1128/mcb.19.2.979
Appears in Collections:
7. Others (기타) > Others (기타) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/173716
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