103 173

Cited 5 times in

Inhibition of miR-449a Promotes Cartilage Regeneration and Prevents Progression of Osteoarthritis in In Vivo Rat Models.

Authors
 Dawoon Baek  ;  Kyoung-Mi Lee  ;  Ki Won Park  ;  Jae Wan Suh  ;  Seong Mi Choi  ;  Kwang Hwan Park  ;  Jin Woo Lee  ;  Sung-Hwan Kim 
Citation
 MOLECULAR THERAPY-NUCLEIC ACIDS, Vol.13 : 322-333, 2018 
Journal Title
 MOLECULAR THERAPY-NUCLEIC ACIDS 
Issue Date
2018
Keywords
cartilage regeneration ; human mesenchymal stem cell ; miR-449a ; microRNA ; osteoarthritis
Abstract
Traumatic and degenerative lesions of articular cartilage usually progress to osteoarthritis (OA), a leading cause of disability in humans. MicroRNAs (miRNAs) can regulate the differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) and play important roles in the expression of genes related to OA. However, their functional roles in OA remain poorly understood. Here, we have examined miR-449a, which targets sirtuin 1 (SIRT1) and lymphoid enhancer-binding factor-1 (LEF-1), and observed its effects on damaged cartilage. The levels of chondrogenic markers and miR-449a target genes increased during chondrogenesis in anti-miR-449a-transfected hBMSCs. A locked nucleic acid (LNA)-anti-miR-449a increased cartilage regeneration and expression of type II collagen and aggrecan on the regenerated cartilage surface in acute defect and OA models. Furthermore, intra-articular injection of LNA-anti-miR-449a prevented disease progression in the OA model. Our study indicates that miR-449a may be a novel potential therapeutic target for age-related joint diseases like OA.
Files in This Item:
T201803773.pdf Download
DOI
10.1016/j.omtn.2018.09.015
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Kim, Sung Hwan(김성환) ORCID logo https://orcid.org/0000-0001-5743-6241
Park, Kwang Hwan(박광환) ORCID logo https://orcid.org/0000-0002-2110-0559
Baek, Dawoon(백다운) ORCID logo https://orcid.org/0000-0002-3794-6153
Lee, Kyoung Mi(이경미) ORCID logo https://orcid.org/0000-0002-9038-8162
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/165287
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse