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NPP1 is responsible for potent extracellular ATP hydrolysis as NTPDase1 in primary cultured murine microglia

Authors
 Hye Min Lim  ;  Woon Heo  ;  Jung Woo Han  ;  Min Goo Lee  ;  Joo Young Kim 
Citation
 Purinergic Signalling, Vol.14(2) : 157-166, 2018 
Journal Title
 Purinergic Signalling 
ISSN
 1573-9538 
Issue Date
2018
Keywords
Ectoenzyme ; Microglia ; Migration ; NPP1 ; NTPDase1 ; Phagocytosis
Abstract
The movement of microglia is regulated mainly by P1 and P2 purinergic receptors, which are activated by various nucleotides and their metabolites. Recently, such purinergic signalling has been spotlighted because of potential roles in the pathophysiologies of neurodegenerative and neuropsychiatric disorders. To understand the characteristics of microglia in relation of P1 and P2 signalling, we investigated the ectoenzymes expressed in microglia. At first, we profiled the expression of all known ectoenzymes in cultured microglia. We found that, like NTPDase1 (ectonucleoside triphosphate diphosphohydrolase 1, CD39), NPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1, PC-1) is also highly expressed in primary cultured murine microglia. Knockdown of NPP1 significantly reduced ATP hydrolysis and Pi production in cultured microglia. In addition, the knockdown of NPP1 enhanced basal nucleotide-stimulating responses of cultured microglia, such as phagocytosis and cell migration, and these results were very similar to NTPDase1 knockdown results. Moreover, inhibition of the adenosine receptors by caffeine treatment reduced phagocytosis of NPP1 knock downed-cultured microglia. In conclusion, we suggest that these potent ectoenzymes of primary cultured murine microglia, NPP1 together with CD73 (ecto-5'-nucleotidase) maintain the adenosine levels for triggering nucleotide-stimulating responses.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/165119
Files in This Item:
T201803650.pdf Download
DOI
10.1007/s11302-018-9601-z
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실)
Yonsei Authors
김주영(Kim, Joo Young) ORCID logo https://orcid.org/0000-0003-2623-1491
이민구(Lee, Min Goo) ORCID logo https://orcid.org/0000-0001-7436-012X
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