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NPP1 is responsible for potent extracellular ATP hydrolysis as NTPDase1 in primary cultured murine microglia

DC Field Value Language
dc.contributor.author김주영-
dc.contributor.author이민구-
dc.date.accessioned2018-11-08T16:40:47Z-
dc.date.available2018-11-08T16:40:47Z-
dc.date.issued2018-
dc.identifier.issn1573-9538-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/165119-
dc.description.abstractThe movement of microglia is regulated mainly by P1 and P2 purinergic receptors, which are activated by various nucleotides and their metabolites. Recently, such purinergic signalling has been spotlighted because of potential roles in the pathophysiologies of neurodegenerative and neuropsychiatric disorders. To understand the characteristics of microglia in relation of P1 and P2 signalling, we investigated the ectoenzymes expressed in microglia. At first, we profiled the expression of all known ectoenzymes in cultured microglia. We found that, like NTPDase1 (ectonucleoside triphosphate diphosphohydrolase 1, CD39), NPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1, PC-1) is also highly expressed in primary cultured murine microglia. Knockdown of NPP1 significantly reduced ATP hydrolysis and Pi production in cultured microglia. In addition, the knockdown of NPP1 enhanced basal nucleotide-stimulating responses of cultured microglia, such as phagocytosis and cell migration, and these results were very similar to NTPDase1 knockdown results. Moreover, inhibition of the adenosine receptors by caffeine treatment reduced phagocytosis of NPP1 knock downed-cultured microglia. In conclusion, we suggest that these potent ectoenzymes of primary cultured murine microglia, NPP1 together with CD73 (ecto-5'-nucleotidase) maintain the adenosine levels for triggering nucleotide-stimulating responses.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherSpringer-
dc.relation.isPartOfPURINERGIC SIGNALLING-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleNPP1 is responsible for potent extracellular ATP hydrolysis as NTPDase1 in primary cultured murine microglia-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학교실)-
dc.contributor.googleauthorHye Min Lim-
dc.contributor.googleauthorWoon Heo-
dc.contributor.googleauthorJung Woo Han-
dc.contributor.googleauthorMin Goo Lee-
dc.contributor.googleauthorJoo Young Kim-
dc.identifier.doi10.1007/s11302-018-9601-z-
dc.contributor.localIdA00942-
dc.contributor.localIdA02781-
dc.relation.journalcodeJ03495-
dc.identifier.eissn1573-9546-
dc.identifier.pmid29516286-
dc.subject.keywordEctoenzyme-
dc.subject.keywordMicroglia-
dc.subject.keywordMigration-
dc.subject.keywordNPP1-
dc.subject.keywordNTPDase1-
dc.subject.keywordPhagocytosis-
dc.contributor.alternativeNameKim, Joo Young-
dc.contributor.alternativeNameLee, Min Goo-
dc.contributor.affiliatedAuthor김주영-
dc.contributor.affiliatedAuthor이민구-
dc.citation.volume14-
dc.citation.number2-
dc.citation.startPage157-
dc.citation.endPage166-
dc.identifier.bibliographicCitationPURINERGIC SIGNALLING, Vol.14(2) : 157-166, 2018-
dc.identifier.rimsid60523-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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