Cited 10 times in
HIF1A overexpression using cell-penetrating DNA-binding protein induces angiogenesis in vitro and in vivo
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 송제선 | - |
dc.contributor.author | 신유석 | - |
dc.contributor.author | 이재훈 | - |
dc.contributor.author | 정의원 | - |
dc.date.accessioned | 2018-08-28T16:43:22Z | - |
dc.date.available | 2018-08-28T16:43:22Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 0300-8177 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/161871 | - |
dc.description.abstract | Hypoxia-inducible factor-1 alpha (HIF1A) is an important transcription factor for angiogenesis. Recent studies have used the protein transduction domain (PTD) to deliver genes, but the PTD has not been used to induce the expression of HIF1A. This study aimed at using a novel PTD (Hph-1-GAL4; ARVRRRGPRR) to overexpress the HIF1A and identify the effects on angiogenesis in vitro and in vivo. Overexpression of HIF1A was induced using Hph-1-GAL4 in human umbilical vein/vascular endothelium cells (HUVEC). The expression levels of genes were analyzed by the quantitative real-time polymerase chain reaction (qPCR) after 2 and 4 days, respectively. An in vitro tube formation was performed using Diff-Quik staining. HIF1A and Hph-1-GAL4 were injected subcutaneously into the ventral area of each 5-week-old mouse. All of the plugs were retrieved after 1 week, and the gene expression levels were evaluated by qPCR. Each Matrigel plug was evaluated using the hemoglobin assay and hematoxylin and eosin (HE) staining. The expression levels of HIF1A and HIF1A target genes were significantly higher in HIF1A-transfected HUVEC than in control HUVEC in vitro. In the in vivo Matrigel plug assay, the amount of hemoglobin was significantly higher in the HIF1A-treatment group than in the PBS-treatment group. Blood vessels were identified in the HIF1A-treatment group. The expression levels of HIF1A, vascular endothelial growth factor (Vegf), and Cd31 were significantly higher in the HIF1A-treatment group than in the PBS-treatment group. These findings suggest that using Hph-1-G4D to overexpress HIF1A might be useful for transferring genes and regenerating tissues. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Kluwer Academic | - |
dc.relation.isPartOf | MOLECULAR AND CELLULAR BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell-Penetrating Peptides/*pharmacology | - |
dc.subject.MESH | Human Umbilical Vein Endothelial Cells/cytology/*metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | alpha Subunit/*biosynthesis Hypoxia-Inducible Factor 1 | - |
dc.subject.MESH | Physiologic/*drug effects Neovascularization | - |
dc.subject.MESH | Platelet Endothelial Cell Adhesion Molecule-1/biosynthesis | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A/biosynthesis | - |
dc.title | HIF1A overexpression using cell-penetrating DNA-binding protein induces angiogenesis in vitro and in vivo | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry | - |
dc.contributor.department | Dept. of Pediatric Dentistry | - |
dc.contributor.googleauthor | Mijeong Jeon | - |
dc.contributor.googleauthor | Yooseok Shin | - |
dc.contributor.googleauthor | Jaeeun Jung | - |
dc.contributor.googleauthor | Ui-Won Jung | - |
dc.contributor.googleauthor | Jae-Hoon Lee | - |
dc.contributor.googleauthor | Jae-Seung Moon | - |
dc.contributor.googleauthor | Ilkoo Kim | - |
dc.contributor.googleauthor | Jin-Su Shin | - |
dc.contributor.googleauthor | Sang-Kyou Lee | - |
dc.contributor.googleauthor | Je Seon Song | - |
dc.identifier.doi | 10.1007/s11010-017-3098-6 | - |
dc.contributor.localId | A02058 | - |
dc.contributor.localId | A02129 | - |
dc.contributor.localId | A03091 | - |
dc.contributor.localId | A03692 | - |
dc.relation.journalcode | J02242 | - |
dc.identifier.eissn | 1573-4919 | - |
dc.identifier.pmid | 28660411 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2Fs11010-017-3098-6 | - |
dc.subject.keyword | Angiogenesis | - |
dc.subject.keyword | HUVEC | - |
dc.subject.keyword | Hypoxia-inducible factor-1 alpha (HIF1A) | - |
dc.subject.keyword | Protein transduction domain (PTD) | - |
dc.subject.keyword | Tissue regeneration | - |
dc.contributor.alternativeName | Song, Je Seon | - |
dc.contributor.alternativeName | Shin, Yoo Seok | - |
dc.contributor.alternativeName | Lee, Jae Hoon | - |
dc.contributor.alternativeName | Jung, Ui Won | - |
dc.contributor.affiliatedAuthor | Song, Je Seon | - |
dc.contributor.affiliatedAuthor | Shin, Yoo Seok | - |
dc.contributor.affiliatedAuthor | Lee, Jae Hoon | - |
dc.contributor.affiliatedAuthor | Jung, Ui Won | - |
dc.citation.volume | 437 | - |
dc.citation.number | 1-2 | - |
dc.citation.startPage | 99 | - |
dc.citation.endPage | 107 | - |
dc.identifier.bibliographicCitation | MOLECULAR AND CELLULAR BIOCHEMISTRY, Vol.437(1-2) : 99-107, 2018 | - |
dc.identifier.rimsid | 59463 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.