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Concordance of programmed death-ligand 1 expression between primary and metastatic non-small cell lung cancer by immunohistochemistry and RNA in situ hybridization

Authors
 Hye Ryun Kim  ;  Yoon Jin Cha  ;  Min Hee Hong  ;  Manoj Gandhi  ;  Shauna Levinson  ;  Inkyung Jung  ;  Jin Gu Lee  ;  Chang Young Lee  ;  Byoung Chul Cho  ;  Sang-Jun Ha  ;  Hyo Sup Shim 
Citation
 Oncotarget, Vol.8(50) : 87234-87243, 2017 
Journal Title
 Oncotarget 
Issue Date
2017
Keywords
RNA in situ hybridization ; immunohistochemistry ; non-small cell lung cancer ; programmed death ligand-1
Abstract
We investigated the concordance of programmed death-ligand 1 (PD-L1) expression between primary cancer at initial diagnosis and metastasis at recurrence in resected non-small cell lung cancer (NSCLC). PD-L1 expression was evaluated using the SP142 assay in 37 NSCLC patients with paired primary lung cancer and surgically resected metastases at recurrence. PD-L1 positivity was defined as immunohistochemistry (IHC) and also evaluated by RNA in situ hybridization (RISH). The concordance rate of PD-L1 between primaries and metastases and correlation with clinicopathological factors were analyzed. PD-L1 expression was higher in squamous cell carcinoma, wild-type EGFR, and smokers than in non-squamous carcinoma, mutant EGFR, and never smokers, respectively. PD-L1 positivity was observed in 18.9% of primaries and 21.6% of metastases. IHC demonstrated 78.4% concordance of PD-L1 positivity between primary and metastatic cancers. In 10.8% of cases, PD-L1 positivity was higher in primaries than in metastases, and vice versa in the remaining 10.8%. By PD-L1 RISH, 35.1% of primaries and 27.0% of metastases demonstrated PD-L1 positivity. There was 62.2% concordance in PD-L1 by RISH between the primaries and metastases. Our results thus highlight the clinical importance of replacing metastases with primary archival tissue, particularly when re-biopsy is difficult at recurrence.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/161573
DOI
10.18632/oncotarget.20254
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Biomedical Systems Informatics (의생명시스템정보학교실)
Yonsei Authors
김혜련(Kim, Hye Ryun) ; 심효섭(Shim, Hyo Sup) ; 이진구(Lee, Jin Gu) ; 이창영(Lee, Chang Young) ; 정인경(Jung, In Kyung) ; 조병철(Cho, Byoung Chul) ; 차윤진(Cha, Yoon Jin) ; 홍민희(Hong, Min Hee)
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