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Concordance of programmed death-ligand 1 expression between primary and metastatic non-small cell lung cancer by immunohistochemistry and RNA in situ hybridization

 Hye Ryun Kim  ;  Yoon Jin Cha  ;  Min Hee Hong  ;  Manoj Gandhi  ;  Shauna Levinson  ;  Inkyung Jung  ;  Jin Gu Lee  ;  Chang Young Lee  ;  Byoung Chul Cho  ;  Sang-Jun Ha  ;  Hyo Sup Shim 
 Oncotarget, Vol.8(50) : 87234-87243, 2017 
Journal Title
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RNA in situ hybridization ; immunohistochemistry ; non-small cell lung cancer ; programmed death ligand-1
We investigated the concordance of programmed death-ligand 1 (PD-L1) expression between primary cancer at initial diagnosis and metastasis at recurrence in resected non-small cell lung cancer (NSCLC). PD-L1 expression was evaluated using the SP142 assay in 37 NSCLC patients with paired primary lung cancer and surgically resected metastases at recurrence. PD-L1 positivity was defined as immunohistochemistry (IHC) and also evaluated by RNA in situ hybridization (RISH). The concordance rate of PD-L1 between primaries and metastases and correlation with clinicopathological factors were analyzed. PD-L1 expression was higher in squamous cell carcinoma, wild-type EGFR, and smokers than in non-squamous carcinoma, mutant EGFR, and never smokers, respectively. PD-L1 positivity was observed in 18.9% of primaries and 21.6% of metastases. IHC demonstrated 78.4% concordance of PD-L1 positivity between primary and metastatic cancers. In 10.8% of cases, PD-L1 positivity was higher in primaries than in metastases, and vice versa in the remaining 10.8%. By PD-L1 RISH, 35.1% of primaries and 27.0% of metastases demonstrated PD-L1 positivity. There was 62.2% concordance in PD-L1 by RISH between the primaries and metastases. Our results thus highlight the clinical importance of replacing metastases with primary archival tissue, particularly when re-biopsy is difficult at recurrence.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Biomedical Systems Informatics (의생명시스템정보학교실) > 1. Journal Papers
Yonsei Authors
김혜련(Kim, Hye Ryun) ORCID logo https://orcid.org/0000-0002-1842-9070
심효섭(Shim, Hyo Sup) ORCID logo https://orcid.org/0000-0002-5718-3624
이진구(Lee, Jin Gu)
이창영(Lee, Chang Young)
정인경(Jung, Inkyung) ORCID logo https://orcid.org/0000-0003-3780-3213
조병철(Cho, Byoung Chul) ORCID logo https://orcid.org/0000-0002-5562-270X
차윤진(Cha, Yoon Jin) ORCID logo https://orcid.org/0000-0002-5967-4064
홍민희(Hong, Min Hee) ORCID logo https://orcid.org/0000-0003-3490-2195
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