0 18

Instantaneous pH-Boosted Functionalization of Stellate Gold Nanoparticles for Intracellular Imaging of miRNA

Authors
 Jisun Ki  ;  Eunji Jang  ;  Seungmin Han  ;  Moo-Kwang Shin  ;  Byunghoon Kang  ;  Yong-Min Huh  ;  Seungjoo Haam 
Citation
 ACS Applied Materials & Interfaces, Vol.9(21) : 17702-17709, 2017 
Journal Title
 ACS Applied Materials & Interfaces 
ISSN
 1944-8244 
Issue Date
2017
Keywords
branched gold nanoparticles ; cancer imaging ; miRNA sensing ; molecular beacon ; nanobiosensor
Abstract
Various types of nanoprobes have recently been utilized to monitor living organisms by detecting and imaging intracellular biomarkers, such as microRNAs (miRs). We here present a simple one-pot method to prepare stellate gold nanoparticles functionalized with miR-detecting molecular beacons (SGNP-MBs); low pH conditions permitted the rapid-high loading of MBs on the surface of SGNPs. Compared to the conventional gold nanoparticle-based MBs, SGNPs carried a 4.5-fold higher load of MBs and exhibited a 6.4-fold higher cellular uptake. We demonstrated that SGNP-MBs were successfully internalized in human gastric cancer cell lines and could be used to accurately detect and image intracellular miRs in an miR-specific manner. Furthermore, the relative levels of intracellular miRs in three different cell lines expressing miR-10b (high, moderate, and low levels) could be monitored using SGNP-MBs. Consequently, these results indicated that SGNP-MBs could have applications as highly potent, efficient nanoprobes to assess intracellular miR levels in living cells.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160997
Full Text
https://pubs.acs.org/doi/10.1021/acsami.6b16452
DOI
10.1021/acsami.6b16452
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실)
Yonsei Authors
허용민(Huh, Yong Min)
Export
RIS (EndNote)
XLS (Excel)
XML
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse