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Instantaneous pH-Boosted Functionalization of Stellate Gold Nanoparticles for Intracellular Imaging of miRNA

Authors
 Jisun Ki  ;  Eunji Jang  ;  Seungmin Han  ;  Moo-Kwang Shin  ;  Byunghoon Kang  ;  Yong-Min Huh  ;  Seungjoo Haam 
Citation
 ACS Applied Materials & Interfaces, Vol.9(21) : 17702-17709, 2017 
Journal Title
 ACS Applied Materials & Interfaces 
ISSN
 1944-8244 
Issue Date
2017
Keywords
branched gold nanoparticles ; cancer imaging ; miRNA sensing ; molecular beacon ; nanobiosensor
Abstract
Various types of nanoprobes have recently been utilized to monitor living organisms by detecting and imaging intracellular biomarkers, such as microRNAs (miRs). We here present a simple one-pot method to prepare stellate gold nanoparticles functionalized with miR-detecting molecular beacons (SGNP-MBs); low pH conditions permitted the rapid-high loading of MBs on the surface of SGNPs. Compared to the conventional gold nanoparticle-based MBs, SGNPs carried a 4.5-fold higher load of MBs and exhibited a 6.4-fold higher cellular uptake. We demonstrated that SGNP-MBs were successfully internalized in human gastric cancer cell lines and could be used to accurately detect and image intracellular miRs in an miR-specific manner. Furthermore, the relative levels of intracellular miRs in three different cell lines expressing miR-10b (high, moderate, and low levels) could be monitored using SGNP-MBs. Consequently, these results indicated that SGNP-MBs could have applications as highly potent, efficient nanoprobes to assess intracellular miR levels in living cells.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/160997
DOI
10.1021/acsami.6b16452
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실)
Yonsei Authors
허용민(Huh, Yong Min)
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Full Text
https://pubs.acs.org/doi/10.1021/acsami.6b16452
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