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Chemokine in inflamed periodontal tissues activates healthy periodontal-ligament stem cell migration

Authors
 Jung‐Seok Lee  ;  Jong‐Bin Lee  ;  Jae‐Kook Cha  ;  Eun‐Young Choi  ;  So‐Yon Park  ;  Kyoo‐Sung Cho  ;  Chang‐Sung Kim 
Citation
 Journal of Clinical Periodontology, Vol.44(5) : 530-539, 2017 
Journal Title
 Journal of Clinical Periodontology 
ISSN
 0303-6979 
Issue Date
2017
Keywords
chemokines ; homing ; migration ; periodontal stem cells ; tissue regen
Abstract
AIM: The present study aimed to characterize the expression pattern of chemokines obtained from inflamed periodontal defects and to determine the characteristics of human periodontal-ligament stem cells (hPDLSCs) migrated by each specific chemokine. MATERIALS AND METHODS: Both inflamed and healthy periodontal tissues were obtained from periodontitis patients (n = 11), and the chemokine expression levels were analyzed. The periodontal-tissue-specific chemokines were applied to healthy hPDLSCs from extracted teeth (n = 3), with FGF-2 acting as a positive control. Cells were separated by selected chemokines using transwell method into migrated/unmigrated hPDLSCs. The characteristics of the hPDLSC subpopulation recruited by each chemokine were assessed, and gene expression pattern was analyzed by microarray. RESULTS: Chemokines were categorized into three groups by specific patterns of "appearing," "increasing," and "decreasing/disappearing" from healthy to inflamed tissues. A representative chemokine from each group enhanced the capacities for colony formation and osteogenic/adipogenic differentiation while maintaining the surface markers of hPDLSCs. RANTES/CCL5 significantly increased the cellular migration of hPDLSCs, via enhancement of signaling pathways, regulation of the actin skeleton, and focal adhesion. CONCLUSION: The present study found a specific chemokine profile induced by inflammation in periodontal tissues, with RANTES/CCL5 appearing to play a role in the migration of hPDLSCs into inflammatory periodontal lesions.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/160937
DOI
10.1111/jcpe.12710
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실)
Yonsei Authors
김창성(Kim, Chang Sung) ; 이중석(Lee, Jung Seok) ; 조규성(Cho, Kyoo Sung) ; 차재국(Cha, Jae Kook)
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Full Text
https://onlinelibrary.wiley.com/doi/abs/10.1111/jcpe.12710
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