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Identification of evidence for autoimmune pathology of bilateral sudden sensorineural hearing loss using proteomic analysis

Authors
 Jeon Mi Lee  ;  Jin Young Kim  ;  Jinwoong Bok  ;  Kyu-Sung Kim  ;  Jae Young Choi  ;  f  ;  Sung Huhn Kim 
Citation
 CLINICAL IMMUNOLOGY, Vol.183 : 24-35, 2017 
Journal Title
 CLINICAL IMMUNOLOGY 
ISSN
 1521-6616 
Issue Date
2017
MeSH
Administration, Oral ; Adrenal Cortex Hormones/therapeutic use ; Adult ; Aged ; Audiometry, Pure-Tone ; Autoantibodies/immunology* ; Autoantigens/immunology* ; Autoimmunity/immunology* ; Blotting, Western ; Case-Control Studies ; Female ; Hearing Loss, Sensorineural/drug therapy ; Hearing Loss, Sensorineural/immunology* ; Hearing Loss, Sensorineural/physiopathology ; Hearing Loss, Sudden/drug therapy ; Hearing Loss, Sudden/immunology* ; Hearing Loss, Sudden/physiopathology ; Humans ; Immunoprecipitation ; Injection, Intratympanic ; Male ; Mass Spectrometry ; Middle Aged ; Proteomics*
Keywords
Autoantibody ; Autoimmune ; Bilateral sudden sensorineural hearing loss ; Inner ear
Abstract
Sudden sensorineural hearing loss (S-SNHL) is an inner ear disorder with an abrupt hearing loss occurring <3days. The pathologic mechanism of the disease remains unclear, although autoimmunity has been regarded as one of the suggested causes, especially in bilateral form. In this study, we aimed to provide evidence for the involvement of autoimmunity in bilateral S-SNHL using proteomic approaches such as ProtoArray®, western blotting, immunoprecipitation, and liquid column mass spectrometry for mass screening of candidate antigens and autoantibodies based on the hypothesis that multiple autoantibodies and target antigens must exist in order for autoimmune bilateral S-SNHL to develop. As the final outcome, we have proven the involvement of autoimmunity in the disease, and investigated the existence of circulating autoantibodies and candidate antigens. These findings could provide basic evidence necessary for the development of diagnostic biomarkers as well as the understanding of the pathological mechanisms underlying bilateral S-SNHL. S-SNHL: sudden sensorineural hearing loss; LC-MS: liquid chromatography-mass spectrometry; MS: mass spectrometry; autoAb: autoantibody; 1-DE: one-dimensional electrophoresis.
Full Text
https://www.sciencedirect.com/science/article/pii/S1521661617300554
DOI
10.1016/j.clim.2017.06.008
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung Huhn(김성헌)
Bok, Jin Woong(복진웅) ORCID logo https://orcid.org/0000-0003-1958-1872
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/160530
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