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TAGLN2 regulates T cell activation by stabilizing the actin cytoskeleton at the immunological synapse

Authors
 Bo-Ra Na  ;  Hye-Ran Kim  ;  Indre Piragyte  ;  Hyun-Mee Oh  ;  Min-Sung Kwon  ;  Uroos Akber  ;  Hyun-Su Lee  ;  Do-Sim Park  ;  Woo Keun Son  ;  Zee-Yong Park  ;  Sin-Hyeog Im  ;  Mun-Chual Rho  ;  Young-Min Hyun  ;  Minsoo Kim  ;  Chang-Duk Jun 
Citation
 Journal of Cell Biology, Vol.209(1) : 143-162, 2015 
Journal Title
 Journal of Cell Biology 
ISSN
 0021-9525 
Issue Date
2015
MeSH
Actin Cytoskeleton/metabolism* ; Actin Depolymerizing Factors/metabolism ; Animals ; Binding, Competitive ; CHO Cells ; Cricetinae ; Cricetulus ; Humans ; Immunological Synapses/metabolism* ; Immunological Synapses/ultrastructure ; Jurkat Cells ; Lymphocyte Activation ; Mice, 129 Strain ; Mice, Inbred C57BL ; Mice, Knockout ; Microfilament Proteins/physiology* ; Muscle Proteins/physiology* ; Protein Binding ; Protein Interaction Domains and Motifs ; Protein Stability
Abstract
The formation of an immunological synapse (IS) requires tight regulation of actin dynamics by many actin polymerizing/depolymerizing proteins. However, the significance of actin stabilization at the IS remains largely unknown. In this paper, we identify a novel function of TAGLN2--an actin-binding protein predominantly expressed in T cells--in stabilizing cortical F-actin, thereby maintaining F-actin contents at the IS and acquiring LFA-1 (leukocyte function-associated antigen-1) activation after T cell receptor stimulation. TAGLN2 blocks actin depolymerization and competes with cofilin both in vitro and in vivo. Knockout of TAGLN2 (TAGLN2(-/-)) reduced F-actin content and destabilized F-actin ring formation, resulting in decreased cell adhesion and spreading. TAGLN2(-/-) T cells displayed weakened cytokine production and cytotoxic effector function. These findings reveal a novel function of TAGLN2 in enhancing T cell responses by controlling actin stability at the IS.
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DOI
10.1083/jcb.201407130
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
현영민(Hyun, Young-Min) ORCID logo https://orcid.org/0000-0002-0567-2039
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/157308
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