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Analysis of miRNA expression patterns in human and mouse hepatocellular carcinoma cells

 Sinhwa Baek  ;  Kyung Joo Cho  ;  Hye-Lim Ju  ;  Hyuk Moon  ;  Sung Hoon Choi  ;  Sook In Chung  ;  Jun Yong Park  ;  Ki Hong Choi  ;  Do Young Kim  ;  Sang Hoon Ahn  ;  Kwang-Hyub Han  ;  Simon Weonsang Ro 
 HEPATOLOGY RESEARCH, Vol.45(13) : 1331-1340, 2015 
Journal Title
Issue Date
hepatocellular carcinoma ; hepatoma cell line ; miRNA ; quantitative reverse transcription polymerase chain reaction ; wound-healing assay
AIM: Hepatocellular carcinoma (HCC), one of the most common malignancies in adults displays aberrant miRNA expression during its pathogenesis. We assessed expression of miRNA in surgically resected human HCC of an early stage and murine HCC with a high malignancy in order to find miRNA overexpressed in HCC regardless of tumor stage and underlying etiology. Further, the role of the deregulated miRNA in HCC pathogenesis was investigated.

METHODS: miRNA were isolated from HCC tissues and surrounding non-tumorous tissues from HCC patients and a murine transgenic model of HCC. A quantitative reverse transcription polymerase chain reaction was performed to determine expression levels of miRNA. Human HCC cell lines stably expressing individual miRNA were generated to investigate the biological function of overexpressed miRNA.

RESULTS: We found that levels of miR-221, -181b-1, -155-5p, -25 and -17-5p were significantly upregulated in both human and murine HCC regardless of tumor stage, underlying etiology or the presence of fibrosis. Using HCC cell lines stably expressing respective miRNA, we found that miR-221 increased the proliferation of hepatoma cells, while miR-17-5p induced cell migration.

CONCLUSION: We identified miRNA that are consistently upregulated in HCC. The overexpressed miRNA could potentially be used as a bona fide biomarker for HCC.
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Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Do Young(김도영)
Ro, Simon Weonsang(노원상) ORCID logo https://orcid.org/0000-0003-2187-3698
Moon, Hyuk(문혁)
Park, Jun Yong(박준용) ORCID logo https://orcid.org/0000-0001-6324-2224
Baek, Sin Hwa(백신화)
Ahn, Sang Hoon(안상훈) ORCID logo https://orcid.org/0000-0002-3629-4624
Chung, Sook In(정숙인) ORCID logo https://orcid.org/0000-0002-7915-9203
Cho, Kyuong Joo(조경주)
Ju, Hye Lim(주혜림)
Choi, Gi Hong(최기홍) ORCID logo https://orcid.org/0000-0002-1593-3773
Han, Kwang-Hyub(한광협) ORCID logo https://orcid.org/0000-0003-3960-6539
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