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Analysis of miRNA expression patterns in human and mouse hepatocellular carcinoma cells

DC FieldValueLanguage
dc.contributor.author김도영-
dc.contributor.author문혁-
dc.contributor.author박준용-
dc.contributor.author백신화-
dc.contributor.author안상훈-
dc.contributor.author정숙인-
dc.contributor.author조경주-
dc.contributor.author주혜림-
dc.contributor.author최기홍-
dc.contributor.author한광협-
dc.contributor.author노원상-
dc.date.accessioned2018-03-26T17:04:52Z-
dc.date.available2018-03-26T17:04:52Z-
dc.date.issued2015-
dc.identifier.issn1386-6346-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/157158-
dc.description.abstractAIM: Hepatocellular carcinoma (HCC), one of the most common malignancies in adults displays aberrant miRNA expression during its pathogenesis. We assessed expression of miRNA in surgically resected human HCC of an early stage and murine HCC with a high malignancy in order to find miRNA overexpressed in HCC regardless of tumor stage and underlying etiology. Further, the role of the deregulated miRNA in HCC pathogenesis was investigated. METHODS: miRNA were isolated from HCC tissues and surrounding non-tumorous tissues from HCC patients and a murine transgenic model of HCC. A quantitative reverse transcription polymerase chain reaction was performed to determine expression levels of miRNA. Human HCC cell lines stably expressing individual miRNA were generated to investigate the biological function of overexpressed miRNA. RESULTS: We found that levels of miR-221, -181b-1, -155-5p, -25 and -17-5p were significantly upregulated in both human and murine HCC regardless of tumor stage, underlying etiology or the presence of fibrosis. Using HCC cell lines stably expressing respective miRNA, we found that miR-221 increased the proliferation of hepatoma cells, while miR-17-5p induced cell migration. CONCLUSION: We identified miRNA that are consistently upregulated in HCC. The overexpressed miRNA could potentially be used as a bona fide biomarker for HCC.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherBlackwell Publishing-
dc.relation.isPartOfHEPATOLOGY RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleAnalysis of miRNA expression patterns in human and mouse hepatocellular carcinoma cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine-
dc.contributor.departmentDept. of Internal Medicine-
dc.contributor.googleauthorSinhwa Baek-
dc.contributor.googleauthorKyung Joo Cho-
dc.contributor.googleauthorHye-Lim Ju-
dc.contributor.googleauthorHyuk Moon-
dc.contributor.googleauthorSung Hoon Choi-
dc.contributor.googleauthorSook In Chung-
dc.contributor.googleauthorJun Yong Park-
dc.contributor.googleauthorKi Hong Choi-
dc.contributor.googleauthorDo Young Kim-
dc.contributor.googleauthorSang Hoon Ahn-
dc.contributor.googleauthorKwang-Hyub Han-
dc.contributor.googleauthorSimon Weonsang Ro-
dc.identifier.doi10.1111/hepr.12510-
dc.contributor.localIdA00385-
dc.contributor.localIdA01391-
dc.contributor.localIdA01675-
dc.contributor.localIdA01828-
dc.contributor.localIdA02226-
dc.contributor.localIdA03640-
dc.contributor.localIdA03804-
dc.contributor.localIdA03961-
dc.contributor.localIdA04046-
dc.contributor.localIdA04268-
dc.contributor.localIdA01286-
dc.relation.journalcodeJ00987-
dc.identifier.eissn1872-034X-
dc.identifier.pmid25704452-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/hepr.12510/abstract-
dc.subject.keywordhepatocellular carcinoma-
dc.subject.keywordhepatoma cell line-
dc.subject.keywordmiRNA-
dc.subject.keywordquantitative reverse transcription polymerase chain reaction-
dc.subject.keywordwound-healing assay-
dc.contributor.alternativeNameKim, Do Young-
dc.contributor.alternativeNameMoon, Hyuk-
dc.contributor.alternativeNamePark, Jun Yong-
dc.contributor.alternativeNameBaek, Sin Hwa-
dc.contributor.alternativeNameAhn, Sang Hoon-
dc.contributor.alternativeNameChung, Sook In-
dc.contributor.alternativeNameCho, Kyuong Joo-
dc.contributor.alternativeNameJu, Hye Lim-
dc.contributor.alternativeNameChoi, Gi Hong-
dc.contributor.alternativeNameHan, Kwang Hyup-
dc.contributor.affiliatedAuthorKim, Do Young-
dc.contributor.affiliatedAuthorMoon, Hyuk-
dc.contributor.affiliatedAuthorPark, Jun Yong-
dc.contributor.affiliatedAuthorBaek, Sin Hwa-
dc.contributor.affiliatedAuthorAhn, Sang Hoon-
dc.contributor.affiliatedAuthorChung, Sook In-
dc.contributor.affiliatedAuthorCho, Kyuong Joo-
dc.contributor.affiliatedAuthorJu, Hye Lim-
dc.contributor.affiliatedAuthorChoi, Gi Hong-
dc.contributor.affiliatedAuthorHan, Kwang Hyup-
dc.contributor.affiliatedAuthorRo, Simon Weonsang-
dc.citation.volume45-
dc.citation.number13-
dc.citation.startPage1331-
dc.citation.endPage1340-
dc.identifier.bibliographicCitationHEPATOLOGY RESEARCH, Vol.45(13) : 1331-1340, 2015-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Liver Cirrhosis Clinical Research Center (간경변증임상연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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