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Characteristics of stem cells from human exfoliated deciduous teeth (SHED) from intact cryopreserved deciduous teeth

Authors
 Hyo-Seol Lee  ;  Mi Jung Jeon  ;  Seong-Oh Kim  ;  Seung-Hye Kim  ;  Jea-Ho Lee  ;  Su-Jin Ahn  ;  Yooseok Shin  ;  Je Seon Song 
Citation
 Cryobiology, Vol.71(3) : 374-383, 2015 
Journal Title
 Cryobiology 
ISSN
 0011-2240 
Issue Date
2015
MeSH
Animals ; Cell Differentiation ; Cryopreservation/methods* ; Flow Cytometry ; Humans ; Mice ; Stem Cells/cytology* ; Tooth, Deciduous/cytology*
Keywords
Cryopreservation ; Deciduous teeth ; Dental pulp stem cells ; Frozen teeth ; Intact teeth ; In vivo ; Mesenchymal stem cells ; SHED ; Stem cells ; Stem cells from human exfoliated deciduous teeth
Abstract
The aim of this study is to compare the characteristics of stem cells derived from human exfoliated deciduous teeth (SHED) from cryopreserved intact deciduous teeth with those of fresh SHED. In total, 20 exfoliated deciduous teeth were randomly divided into a fresh group (f-SHED; n = 11) and cryopreserved group (c-SHED; n = 9; stored for 1-8 months). Following thawing and separation of the pulp, the SHED cells were cultured, and the characteristics as mesenchymal stem cells were investigated using proliferation assays, cell-cycle analysis, colony-forming unit-fibroblast (CFU-F) assays, and flow cytometry analyses. Furthermore, differentiation into adipogenic and osteogenic lineages was investigated in vitro as well as in vivo via transplantation in mice. We found no significant differences between the two groups in the proliferation analyses, in the expression of mesenchymal stem cell markers, or in the adipogenic and osteogenic differentiation in vitro (p < 0.05). Furthermore, the in vivo transplantation results showed no significant differences in the quantity of bone tissue that formed or in histochemistry performance (p < 0.05). In conclusion, cryopreservation of intact exfoliated deciduous teeth appears to be a useful method for preserving SHED.
Full Text
http://www.sciencedirect.com/science/article/pii/S001122401500396X
DOI
10.1016/j.cryobiol.2015.10.146
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Science Research Center (구강과학연구소) > 1. Journal Papers
Yonsei Authors
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Kim, Seung Hye(김승혜)
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Shin, Yoo Seok(신유석) ORCID logo https://orcid.org/0000-0003-1121-2570
Lee, Jae Ho(이제호) ORCID logo https://orcid.org/0000-0002-1556-3485
Jeon, Mi Jeong(전미정)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/157132
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