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PGC-Enriched miRNAs Control Germ Cell Development

Authors
 Jinhyuk Bhin  ;  Hoe-Su Jeong  ;  Jong Soo Kim  ;  Jeong Oh Shin  ;  Ki Sung Hong  ;  Han-Sung Jung  ;  Changhoon Kim  ;  Daehee Hwang  ;  Kye-Seong Kim 
Citation
 MOLECULES AND CELLS, Vol.38(10) : 895-903, 2015 
Journal Title
MOLECULES AND CELLS
ISSN
 1016-8478 
Issue Date
2015
MeSH
Animals ; Blotting, Northern ; Cell Line ; Embryo, Mammalian/metabolism* ; Female ; Gene Expression Profiling ; Germ Cells/growth & development* ; Germ Cells/metabolism ; Male ; Mice ; Mice, Inbred ICR ; MicroRNAs/genetics* ; MicroRNAs/physiology ; Oligonucleotide Array Sequence Analysis ; RNA, Messenger/genetics* ; RNA, Messenger/physiology ; Reverse Transcriptase Polymerase Chain Reaction ; Testis/metabolism*
Keywords
bioinformatic analysis ; in situ hybridization ; male primordial germ cells ; miRNAs ; microarray
Abstract
Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unclear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, -106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysis in PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through which these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development.
Files in This Item:
T201504761.pdf Download
DOI
10.14348/molcells.2015.0146
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Jeong Oh(신정오) ORCID logo https://orcid.org/0000-0002-6935-0936
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/156846
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