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Immunomodulatory/anti-inflammatory effect of ZOE-based dental materials

 Jung-Hwan Lee  ;  Hae-Hyoung Lee  ;  Hae-Won Kim  ;  Je-Wook Yue  ;  Kyoung-Nam Kim  ;  Kwang-Mahn Kim 
 DENTAL MATERIALS, Vol.33(1) : 1-12, 2017 
Journal Title
Issue Date
Animals ; Dental Pulp/cytology ; Dental Pulp/immunology* ; Eugenol/toxicity* ; Humans ; Inflammation* ; Mice ; Root Canal Filling Materials/toxicity* ; Zinc Oxide-Eugenol Cement
Eugenol ; Human dental pulp cells ; Immortalized mouse bone marrow monocyte ; Immunomodulatory/anti-inflammatory ; Zinc ion ; Zinc oxide eugenol
OBJECTIVE: The study assessed the cytotoxicity and immunomodulatory/anti-inflammatory effect of extract from zinc oxide-eugenol (ZOE)-based dental materials during setting using immortalized human dental pulp stem cells (IHDPSCs) and mouse bone marrow monocytes (IMBMMs), and identified the responsible extract component.

METHODS: In accord with the ISO 10993-12, we extracted a mixture of ZOE cement and sealer after a specified time. The extract was analyzed by two types of mass spectrometry (ICP-MS and GC-MS). Cell viability was evaluated with extract and serial concentrations of ZnCl2, ZnSO4, and eugenol liquid by WST assay. The immunomodulatory/anti-inflammatory effect of a ZOE component was determined by RT-PCR to detect the downregulatory effect of inflammatory mRNA expression after lipopolysaccharide (LPS)-induced inflammation.

RESULTS: Zn2+ and eugenol (2-20ppm) were detected in the ZOE cement and sealer extracts. During the early stage of setting, significant cytotoxicity was observed in IHDPSCs and IMBMMs (p<0.05). The half maximal effective concentration of Zn2+ was 5-8ppm, whereas that of eugenol could not be detected within 80ppm. After extract treatment, the expression of inflammatory mRNA was significantly lower in inflamed IHDPSCs, but not inflamed IMBMMs, than in the LPS control (p<0.05). However, eugenol, not Zn2+, at 5-20ppm downregulated inflammatory mRNA expression in the inflamed IMBMMs with and without the exchange of LPS-pretreated medium.

SIGNIFICANCE: ZOE was highly cytotoxic, especially during setting, to both cells due to Zn2+ while the immunomodulatory/anti-inflammatory effect of ZOE was induced by eugenol.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyoung Nam(김경남)
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
Yu, Je Wook(유제욱) ORCID logo https://orcid.org/0000-0001-5943-4071
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