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Role of TGFBIp in Wound Healing and Mucin Expression in Corneal Epithelial Cells

 Yong-Sun Maeng  ;  Ga-Hyun Lee  ;  Boram Lee  ;  Seung-Il Choi  ;  Tae-im Kim  ;  Eung Kweon Kim 
 Yonsei Medical Journal, Vol.58(2) : 423-431, 2017 
Journal Title
 Yonsei Medical Journal 
Issue Date
Blotting, Western ; Cell Adhesion ; Cell Movement/drug effects ; Cell Proliferation ; Cells, Cultured ; Conjunctiva/cytology ; Cornea/cytology* ; Epithelial Cells/metabolism* ; Extracellular Matrix Proteins/physiology* ; Gene Expression ; Humans ; Mucins/genetics ; Mucins/metabolism* ; Signal Transduction ; Transforming Growth Factor beta/physiology* ; Wound Healing/physiology*
TGFBIp ; cornea ; epithelial cells ; mucin
PURPOSE: Transforming growth factor-β-induced protein (TGFBIp) is highly expressed in the cornea, and mutant TGFBIp induces corneal diseases. However, the function of TGFBIp in cornea epithelium is not fully investigated. Here, we tested the importance of TGFBIp in regulation of gene expression and corneal epithelial cell (CEC) activity. MATERIALS AND METHODS: The effect of TGFBIp on CEC activity was analyzed by cell migration, adhesion, proliferation and wound healing assay. Analysis of gene expression was examined by western blot and quantitative reverse transcription PCR. RESULTS: The results demonstrated that TGFBIp increased adhesion, migration, proliferation, and wound healing of CECs. Analysis of gene expression presented that TGFBIp-stimulated CECs exhibited increased expression of mucin family genes, such as MUC1, -4, -5AC, and -16. Furthermore, TGFBIp treatment increased the expression of MUC1, -4, -5AC, -7, and -16 in conjunctival epithelial cells. TGFBIp also increased the activity of intracellular signaling molecules ERK and AKT in CECs. Using pharmacologic inhibitors of ERK and AKT, we showed that the expression of mucin genes by TGFBIp is mediated by the activation of ERK and AKT signaling. CONCLUSION: Our findings demonstrate that the locally generated TGFBIp in the cornea may contribute to wound healing of CECs by enhancing the migration, adhesion, and proliferation of CECs. In addition, our results suggest that TGFBIp has a protective effect on ocular surfaces by inducing the expression of mucin genes in corneal and conjunctival epithelial cells. These data suggest that TGFBIp is a useful therapeutic target for patients with corneal wounds.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Corneal Dystrophy Research Institute (각막이상증연구소) > 1. Journal Papers
Yonsei Authors
김응권(Kim, Eung Kweon) ORCID logo https://orcid.org/0000-0002-1453-8042
김태임(Kim, Tae Im) ORCID logo https://orcid.org/0000-0001-6414-3842
맹용선(Maeng, Yong Sun) ORCID logo https://orcid.org/0000-0003-1694-8405
최승일(Choi, Seung Il) ORCID logo https://orcid.org/0000-0001-7168-8795
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