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Effects for Sequential Treatment of siAkt and Paclitaxel on Gastric Cancer Cell Lines

Authors
 Minhee Ku  ;  Myounghwa Kang  ;  Jin-Suck Suh  ;  Jaemoon Yang 
Citation
 INTERNATIONAL JOURNAL OF MEDICAL SCIENCES, Vol.13(9) : 708-716, 2016 
Journal Title
 INTERNATIONAL JOURNAL OF MEDICAL SCIENCES 
Issue Date
2016
MeSH
Apoptosis/drug effects ; Cell Line, Tumor ; Cell Proliferation/drug effects* ; Cell Survival/drug effects ; Drug Resistance, Neoplasm/genetics ; Drug Synergism ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; Paclitaxel/administration & dosage* ; Proto-Oncogene Proteins c-akt/antagonists & inhibitors ; Proto-Oncogene Proteins c-akt/biosynthesis* ; RNA, Small Interfering/administration & dosage ; Signal Transduction/drug effects ; Stomach Neoplasms/drug therapy* ; Stomach Neoplasms/genetics ; Stomach Neoplasms/pathology
Keywords
Akt ; gastric cancer ; paclitaxel (PTX) ; real-time cell analysis (RTCA) ; sequential treatment ; small interfering RNA (siRNA).
Abstract
Real-time screening of cellular response on the drugs could provide valuable insights for the early detection of therapeutic efficiency and the evaluation of disease progression. Cancer cells have the ability to vary widely in response to stress in a manner to adjust the signaling pathway to promote the survival or having a resistance to stimulation. Cell-based label-free technologies using electronic impedance sensor have strategies for constructing the signature profiles of each cells. To achieve exquisite sensitivity to substantially change of live-cell response have an important role that predict the potential of therapeutic effects. In this study, we use an impedance-based real-time cell analysis system to investigate dynamic phenotypes of cells described as a cellular index value. We show that gastric cancer cells generated characteristic kinetic patterns that corresponded to the treatment order of therapeutics. The kinetic feature of the cells offers insightful information that cannot be acquired from a conventional single end-point assay. Furthermore, we employ a 'sequential treatment strategy' to increase cytotoxic effects with minimizing the use of chemotherapeutics. Specifically, treatment of paclitaxel (PTX) after down-regulating Akt gene expression using RNAi reduces the cell proliferation and increases apoptosis. We propose that the sequential treatment may exhibit more effective approach rather than traditional combination therapy. Moreover, the dynamic monitoring of cell-drug interaction enables us to obtain a better understanding of the temporal effects in vitro
Files in This Item:
T201604253.pdf Download
DOI
10.7150/ijms.15501
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Ku, Min Hee(구민희) ORCID logo https://orcid.org/0000-0002-1674-1474
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/152369
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