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Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0oC/2 Mpa Condition up to One Week: In Vivo MTT Method

 Jang Sun Mi  ;  Cho Sin-Yeon  ;  Kim Eui-Seong  ;  Jung Il-Young  ;  Lee Seung Jong 
 Journal of Korean Dental Science, Vol.9(1) : 1-8, 2016 
Journal Title
Journal of Korean Dental Science
Issue Date
Purpose: The aim of this study was to evaluate the rat periodontal ligament cell viability under $0^{\circ}C/2$ MPa condition up to one week using in vivo 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay. Materials and Methods: As soon as 110 upper molar teeth of rats were extracted, they were stored in Hartman's solution under $0^{\circ}C/2$ MPa condition for 1, 2, 3, 4, and 7 days each. All specimens were treated with in vivo MTT assay and the value of optical density was measured by ELISA reader. These values were statistically analyzed by one-way ANOVA. Result: There was no statistical difference on MTT value between immediate and 1 day storage group. There were statistically significant differences between 1 day and 2 days tsorage, 2 and 3 days storage groups, respectively. Teeth of 34, and 7 days storage groups showed significantly lower MTT valuesc ompared with shorter period storage groups. Conclusion: When the MTT values were substituted in standard curve, 1 day storage group at $0^{\circ}C/2$ MPa condition showed 68% cell viability when compared with immediate group. It dropped to 13% at 2 days, and to less than 5% at 3 days or more.
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2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eui Seong(김의성) ORCID logo https://orcid.org/0000-0003-2126-4761
Lee, Seung Jong(이승종)
Jang, Sun Mi(장선미)
Jung, Il Young(정일영) ORCID logo https://orcid.org/0000-0001-8972-2664
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