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Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0oC/2 Mpa Condition up to One Week: In Vivo MTT Method
DC Field | Value | Language |
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dc.contributor.author | 김의성 | - |
dc.contributor.author | 이승종 | - |
dc.contributor.author | 장선미 | - |
dc.contributor.author | 정일영 | - |
dc.date.accessioned | 2017-10-26T07:09:26Z | - |
dc.date.available | 2017-10-26T07:09:26Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 2005-4742 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/151675 | - |
dc.description.abstract | Purpose: The aim of this study was to evaluate the rat periodontal ligament cell viability under $0^{\circ}C/2$ MPa condition up to one week using in vivo 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay. Materials and Methods: As soon as 110 upper molar teeth of rats were extracted, they were stored in Hartman's solution under $0^{\circ}C/2$ MPa condition for 1, 2, 3, 4, and 7 days each. All specimens were treated with in vivo MTT assay and the value of optical density was measured by ELISA reader. These values were statistically analyzed by one-way ANOVA. Result: There was no statistical difference on MTT value between immediate and 1 day storage group. There were statistically significant differences between 1 day and 2 days tsorage, 2 and 3 days storage groups, respectively. Teeth of 34, and 7 days storage groups showed significantly lower MTT valuesc ompared with shorter period storage groups. Conclusion: When the MTT values were substituted in standard curve, 1 day storage group at $0^{\circ}C/2$ MPa condition showed 68% cell viability when compared with immediate group. It dropped to 13% at 2 days, and to less than 5% at 3 days or more. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | Korean Academy of Dental Sciences | - |
dc.relation.isPartOf | Journal of Korean Dental Science | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0oC/2 Mpa Condition up to One Week: In Vivo MTT Method | - |
dc.type | Article | - |
dc.publisher.location | Korea | - |
dc.contributor.college | College of Dentistry | - |
dc.contributor.department | Dept. of Conservative Dentistry | - |
dc.contributor.googleauthor | Jang Sun Mi | - |
dc.contributor.googleauthor | Cho Sin-Yeon | - |
dc.contributor.googleauthor | Kim Eui-Seong | - |
dc.contributor.googleauthor | Jung Il-Young | - |
dc.contributor.googleauthor | Lee Seung Jong | - |
dc.identifier.doi | 10.5856/JKDS.2016.9.1.1 | - |
dc.contributor.localId | A02925 | - |
dc.contributor.localId | A05027 | - |
dc.contributor.localId | A03700 | - |
dc.contributor.localId | A00833 | - |
dc.relation.journalcode | J01507 | - |
dc.contributor.alternativeName | Kim, Eui Seong | - |
dc.contributor.alternativeName | Lee, Seung Jong | - |
dc.contributor.alternativeName | Jang, Sun Mi | - |
dc.contributor.alternativeName | Jung, Il Young | - |
dc.contributor.affiliatedAuthor | Lee, Seung Jong | - |
dc.contributor.affiliatedAuthor | Jang, Sun Mi | - |
dc.contributor.affiliatedAuthor | Jung, Il Young | - |
dc.contributor.affiliatedAuthor | Kim, Eui Seong | - |
dc.citation.volume | 9 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 8 | - |
dc.identifier.bibliographicCitation | Journal of Korean Dental Science, Vol.9(1) : 1-8, 2016 | - |
dc.date.modified | 2017-10-24 | - |
dc.identifier.rimsid | 45689 | - |
dc.type.rims | ART | - |
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