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Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0oC/2 Mpa Condition up to One Week: In Vivo MTT Method

DC Field Value Language
dc.contributor.author김의성-
dc.contributor.author이승종-
dc.contributor.author장선미-
dc.contributor.author정일영-
dc.date.accessioned2017-10-26T07:09:26Z-
dc.date.available2017-10-26T07:09:26Z-
dc.date.issued2016-
dc.identifier.issn2005-4742-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/151675-
dc.description.abstractPurpose: The aim of this study was to evaluate the rat periodontal ligament cell viability under $0^{\circ}C/2$ MPa condition up to one week using in vivo 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay. Materials and Methods: As soon as 110 upper molar teeth of rats were extracted, they were stored in Hartman's solution under $0^{\circ}C/2$ MPa condition for 1, 2, 3, 4, and 7 days each. All specimens were treated with in vivo MTT assay and the value of optical density was measured by ELISA reader. These values were statistically analyzed by one-way ANOVA. Result: There was no statistical difference on MTT value between immediate and 1 day storage group. There were statistically significant differences between 1 day and 2 days tsorage, 2 and 3 days storage groups, respectively. Teeth of 34, and 7 days storage groups showed significantly lower MTT valuesc ompared with shorter period storage groups. Conclusion: When the MTT values were substituted in standard curve, 1 day storage group at $0^{\circ}C/2$ MPa condition showed 68% cell viability when compared with immediate group. It dropped to 13% at 2 days, and to less than 5% at 3 days or more.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.languageEnglish-
dc.publisherKorean Academy of Dental Sciences-
dc.relation.isPartOfJournal of Korean Dental Science-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleEvaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0oC/2 Mpa Condition up to One Week: In Vivo MTT Method-
dc.typeArticle-
dc.publisher.locationKorea-
dc.contributor.collegeCollege of Dentistry-
dc.contributor.departmentDept. of Conservative Dentistry-
dc.contributor.googleauthorJang Sun Mi-
dc.contributor.googleauthorCho Sin-Yeon-
dc.contributor.googleauthorKim Eui-Seong-
dc.contributor.googleauthorJung Il-Young-
dc.contributor.googleauthorLee Seung Jong-
dc.identifier.doi10.5856/JKDS.2016.9.1.1-
dc.contributor.localIdA02925-
dc.contributor.localIdA05027-
dc.contributor.localIdA03700-
dc.contributor.localIdA00833-
dc.relation.journalcodeJ01507-
dc.contributor.alternativeNameKim, Eui Seong-
dc.contributor.alternativeNameLee, Seung Jong-
dc.contributor.alternativeNameJang, Sun Mi-
dc.contributor.alternativeNameJung, Il Young-
dc.contributor.affiliatedAuthorLee, Seung Jong-
dc.contributor.affiliatedAuthorJang, Sun Mi-
dc.contributor.affiliatedAuthorJung, Il Young-
dc.contributor.affiliatedAuthorKim, Eui Seong-
dc.citation.volume9-
dc.citation.number1-
dc.citation.startPage1-
dc.citation.endPage8-
dc.identifier.bibliographicCitationJournal of Korean Dental Science, Vol.9(1) : 1-8, 2016-
dc.date.modified2017-10-24-
dc.identifier.rimsid45689-
dc.type.rimsART-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers

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