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표피성장인자가 치주인대 섬유 모세포의 증식과 부착에 미치는 영향

Other Titles
 The effects of EGF on proliferation and attachment of humanperiodontal fibroblasts 
Authors
 이종은  ;  김종관  ;  김성오  ;  김연태  ;  최형준 
Citation
 Journal of the Korean Academy of Pediatric Dentistry (대한소아치과학회지), Vol.32(3) : 395-402, 2005 
Journal Title
Journal of the Korean Academy of Pediatric Dentistry(대한소아치과학회지)
ISSN
 1226-8496 
Issue Date
2005
MeSH
EGF ; Fibronectin ; PDL fibroblasts ; Tooth avulsion
Keywords
EGF ; Fibronectin ; PDL fibroblasts ; Tooth avulsion
Abstract
In considering the healing process of injured periodontal tissue, healing rate would be influenced by the cellular activity of periodontal fibroblasts(PDLFs). In addition, the reattachment among PDLFs should be induced for healing process. The purpose of this study was to evaluate the effects of epidermal growth factor(EGF) on the proliferation and attachment of PDLFs and to verify the efficacy of EGF as a storage media or a pre-replantation conditioner of traumatically avulsed tooth. Human recombinant epidermal growth factor(hrEGF) and human periodontal fibroblasts from first premolar were prepared. At first, MTT assay was done to evaluate the toxic effect on human periodontal fibroblast and the maximum cellular growth of EGF. Cellular proliferation rate was then compared between control group and 10ng/ml EGF added group. Also, western blot was done to evaluate the expression of fibronectin in both groups. The results were as follows: 1. From MTT assay, EGF showed no toxic effect on PDL fibroblasts. The highest proliferation was shown at 10ng/ml EGF. 2. In 10ng/ml EGF added group, the degree of proliferation of PDLFs was significantly higher than that in control group. 3. Fibronectin expression of EGF added group was also significantly higher than that of control group. From this study we could conclude that EGF enhanced the regeneration rate of periodontal fibroblast, which could be used as a pretreatment agent or a storage media for traumatically avulsed teeth.
Files in This Item:
T200501224.pdf Download
DOI
OAK-2005-05757
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Kim, Chong Kwan(김종관)
Choi, Hyung Jun(최형준) ORCID logo https://orcid.org/0000-0002-3315-6912
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151388
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