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Effects of Chitosan on Human Periodontal Ligament Fibroblasts In Vitro and on Bone Formation in Rat Calvarial Defects

Authors
 Eun-Kyoung Pang  ;  Jeong-Won Paik  ;  Soo-Kyoung Kim  ;  Ui-Won Jung  ;  Chang-Sung Kim  ;  Kyoo-Sung Cho  ;  Chong-Kwan Kim  ;  Seong-Ho Choi 
Citation
 JOURNAL OF PERIODONTOLOGY, Vol.76(9) : 1526-1533, 2005 
Journal Title
 JOURNAL OF PERIODONTOLOGY 
ISSN
 0022-3492 
Issue Date
2005
MeSH
Animals ; Biocompatible Materials/pharmacology* ; Bone Regeneration/drug effects* ; Chitosan/pharmacology* ; Fibroblasts/drug effects ; Humans ; Male ; Periodontal Ligament/cytology ; Periodontal Ligament/drug effects* ; Rats ; Rats, Sprague-Dawley
Keywords
Animal studies ; bone regeneration ; chitosan ; collagen ; fibroblasts, periodontal ; osteogenesis ; skull
Abstract
BACKGROUND: The purpose of this study was to evaluate the effect of chitosan on human periodontal ligament fibroblasts (hPDLF) in vitro and on bone formation in rat calvarial defects in vivo. METHODS: Fibroblast populations were obtained from individuals with a healthy periodontium and cultured in alpha minimum essential medium (MEM) for the control group. For the experimental groups, cells were cultured in alpha-MEM containing chitosan at concentrations of 0.01, 0.1, 1, or 2 mg/ml. The 3-(4,5-dimethyl-thiazole-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, reverse transcription-polymerase chain reaction (RT-PCR) and the assay of alkaline phosphatase (ALPase) activity were performed. Eight mm calvarial critical-sized defects were created in 30 male Sprague-Dawley rats. The animals were divided into three groups of 10 animals each. The defects were treated with either chitosan/absorbable collagen sponge (ACS) or ACS alone in the experimental groups or were left untreated (surgical controls). The animals were sacrificed at 2 or 8 weeks post-surgery and the treatment outcomes were evaluated using histological and histomorphometric parameters. RESULTS: The chitosan-induced proliferative responses of the hPDLF reached a plateau at a concentration of 0.1 mg/ml (P <0.05). When the hPDLF were stimulated with 0.1 mg/ml chitosan, both the mRNA expression of type I collagen and the ALP activity were significantly up-regulated (P <0.05). The surgical implantation of chitosan/ACS enhanced the new bone formation at 8 weeks post-surgery and the amount of new bone formation of the chitosan/ACS group was significantly greater than that of both the ACS alone group and the surgical control group (P <0.01). The new bone area and defect closure in the chitosan/ACS group were significantly greater than those in the ACS control and sham surgery control groups at 8 weeks (P <0.01). However, the chitosan/ ACS group exhibited significantly less bone density than both the ACS control and the sham surgery control group at 8 weeks (P <0.01). CONCLUSIONS: Chitosan (0.1 mg/ml) enhanced the type I collagen synthesis and facilitated the differentiation into osteogenic cells. Chitosan reconstituted with ACS has a significant potential to accelerate the regeneration of bone in rat calvarial critical size defects.
Full Text
http://www.joponline.org/doi/abs/10.1902/jop.2005.76.9.1526
DOI
10.1902/jop.2005.76.9.1526
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chong Kwan(김종관)
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Paik, Jeong Won(백정원) ORCID logo https://orcid.org/0000-0002-5554-8503
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/151302
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