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Ectopic bone formation associated with recombinant human bone morphogenetic proteins-2 using absorbable collagen sponge and beta tricalcium phosphate as carriers

Authors
 Chang-Sung Kim  ;  Joon-Il Kim  ;  Jin Kim  ;  Seong-Ho Choi  ;  Jung-Kiu Chai  ;  Chong-Kwan Kim  ;  Kyoo-Sung Cho 
Citation
 BIOMATERIALS, Vol.26(15) : 2501-2507, 2005 
Journal Title
 BIOMATERIALS 
ISSN
 0142-9612 
Issue Date
2005
MeSH
Absorbable Implants ; Absorption ; Animals ; Biocompatible Materials/chemistry ; Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins/administration & dosage* ; Bone Substitutes/administration & dosage ; Bone Substitutes/chemistry* ; Bone and Bones/cytology ; Bone and Bones/drug effects* ; Calcium Phosphates/chemistry* ; Choristoma/pathology ; Collagen/chemistry* ; Drug Carriers/chemistry* ; Drug Implants/administration & dosage ; Male ; Materials Testing ; Osteogenesis/drug effects* ; Rats ; Rats, Sprague-Dawley ; Tissue Engineering ; Transforming Growth Factor beta/administration & dosage*
Keywords
Bone morphogenetic proteins ; Ectopic bone formation ; Carrier ; Bone tissue engineering
Abstract
The ectopic bone formation of recombinant human bone morphogenetic protein-2(rhBMP-2) was evaluated using absorbable collagen sponges (ACS) and beta tricalcium phosphate (β-TCP) as carriers in a rat subcutaneous assay model. Subcutaneous pockets were created on the back of rats. The pockets were implanted with rhBMP-2/ACS, rhBMP-2/β-TCP, ACS alone, and β-TCP alone. The rats were sacrificed at 2 or 8 weeks for histological and immunohistochemical evaluation. At 2 weeks, bone formation was evident in both the rhBMP-2/ACS and rhBMP-2/β-TCP sites. At 8 weeks, the quantity of the new bone with a more advanced stage of remodeling had increased further in the rhBMP-2/β-TCP sites. However, the newly formed bone observed at 2 weeks was not found in the rhBMP-2/ACS sites. On immunohistochemical observation, osteopontin staining was observed on both the rhBMP-2/ACS (2 weeks) and rhBMP-2/β-TCP (2 and 8 weeks) sites. Osteocalcin was not detected in any of the samples. The lack of space-providing capacity of ACS may be one of the major factors responsible for its failure to maintain the newly induced bone. Therefore, a carrier for BMPs should provide space for bone formation and maturation during the more advanced healing stages.
Full Text
http://www.sciencedirect.com/science/article/pii/S0142961204006659
DOI
10.1016/j.biomaterials.2004.07.015
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chong Kwan(김종관)
Kim, Jin(김진)
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
Chai, Jung Kyu(채중규)
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/147553
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