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Small-diameter blood vessels engineered with bone marrow-derived cells

 Seung-Woo Cho  ;  Sang Hyun Lim  ;  Il-Kwon Kim  ;  Yoo Sun Hong  ;  Sang-Soo Kim  ;  Kyung Jong Yoo  ;  Hyun-Young Park  ;  Yangsoo Jang  ;  Byung Chul Chang  ;  Cha Yong Choi  ;  Ki-Chul Hwang  ;  Byung-Soo Kim 
 ANNALS OF SURGERY, Vol.241(3) : 506-515, 2005 
Journal Title
Issue Date
Animals ; Bone Marrow Cells*/cytology ; Bone Marrow Cells*/physiology ; Carotid Arteries/physiology ; Carotid Arteries/transplantation* ; Cell Differentiation ; Cells, Cultured ; Dogs ; Endothelium, Vascular/cytology* ; Graft Survival ; Muscle, Smooth, Vascular/cytology* ; Regeneration ; Tissue Engineering* ; Vascular Patency
OBJECTIVE: The objective of this study is to investigate if bone marrow-derived cells (BMCs) regenerate vascular tissues and improve patency in tissue-engineered small-diameter (internal diameter = 3 mm) vascular grafts. SUMMARY BACKGROUND DATA: BMCs have demonstrated the ability to differentiate into endothelial-like cells and vascular smooth muscle-like cells and may offer an alternative cell source for vascular tissue engineering. Thus, we tissue-engineered small-diameter vascular grafts with BMCs and decellularized arteries. METHODS: Canine BMCs were differentiated in vitro into smooth muscle alpha-actin/smooth muscle myosin heavy-chain-positive cells and von Willebrand factor/CD31-positive cells and seeded onto decellularized canine carotid arteries (internal diameter = 3 mm). The seeded grafts were implanted in cell donor dogs. The vascular-tissue regeneration and graft patency were investigated with immunohistochemistry and angiography, respectively. RESULTS: The vascular grafts seeded with BMCs remained patent for up to 8 weeks in the canine carotid artery interposition model, whereas nonseeded grafts occluded within 2 weeks. Within 8 weeks after implantation, the vascular grafts showed regeneration of the 3 elements of artery (endothelium, media, and adventitia). BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular grafts, indicating that the BMCs participated in the vascular tissue regeneration. CONCLUSIONS: Here we show that BMCs have the potential to regenerate vascular tissues and improve patency in tissue-engineered small-diameter vascular grafts. This is the first report of a small-diameter neovessel engineered with BMCs as a cell source.
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1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Yonsei Cardiovascular Research Institute (심혈관연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Il Kwon(김일권)
Yoo, Kyung Jong(유경종) ORCID logo https://orcid.org/0000-0002-9858-140X
Lim, Sang Hyun(임상현)
Chang, Byung Chul(장병철)
Jang, Yang Soo(장양수) ORCID logo https://orcid.org/0000-0002-2169-3112
Hong, You Sun(홍유선)
Hwang, Ki Chul(황기철)
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