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Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol

Authors
 Dong-Wook Han  ;  Hak Hee Kim  ;  Mi Hee Lee  ;  Hyun Sook Baek  ;  Kwon-Yong Lee  ;  Suong-Hyu Hyon  ;  Jong-Chul Park 
Citation
 Biotechnology Letters, Vol.27(9) : 655-660, 2005 
Journal Title
 Biotechnology Letters 
ISSN
 0141-5492 
Issue Date
2005
MeSH
Alkaline Phosphatase/metabolism ; Animals ; Cell Line, Tumor ; Cell Proliferation ; Cryopreservation ; Dimethyl Sulfoxide/chemistry ; Flavonoids/chemistry ; Flavonoids/metabolism ; Freezing ; Guanosine Triphosphate/metabolism ; Humans ; Osteoblasts/cytology* ; Osteoblasts/metabolism ; Oxidative Stress ; Phenols/chemistry ; Phenols/metabolism ; Polyphenols ; Rats ; Rats, Sprague-Dawley ; Reactive Oxygen Species ; Tea
Keywords
green tea polyphenol ; multiple freeze/thaw cycles ; osteoblastic cells ; oxidative stress
Abstract
Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 μg GTP ml−1 significantly (p < 0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.
Full Text
http://link.springer.com/article/10.1007%2Fs10529-005-3677-6
DOI
10.1007/s10529-005-3677-6
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hak Hee(김학희)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Baek, Hyun Sook(백현숙)
Lee, Mi Hee(이미희) ORCID logo https://orcid.org/0000-0002-9630-7044
Han, Dong Wook(한동욱)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/147276
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