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Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol

DC FieldValueLanguage
dc.contributor.author김학희-
dc.contributor.author박종철-
dc.contributor.author백현숙-
dc.contributor.author이미희-
dc.contributor.author한동욱-
dc.date.accessioned2017-05-04T07:25:42Z-
dc.date.available2017-05-04T07:25:42Z-
dc.date.issued2005-
dc.identifier.issn0141-5492-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/147276-
dc.description.abstractGreen tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 μg GTP ml−1 significantly (p < 0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherKluwer Academic Publishers-
dc.relation.isPartOfBiotechnology Letters-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleProtection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol-
dc.typeArticle-
dc.publisher.locationNetherlands-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeOthers-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.departmentDept. of Medical Engineering (의학공학교실)-
dc.contributor.departmentSeverance Hospital (세브란스병원)-
dc.contributor.departmentDept. of Medical Engineering (의학공학교실)-
dc.contributor.departmentDept. of Medical Engineering (의학공학교실)-
dc.contributor.googleauthorDong-Wook Han-
dc.contributor.googleauthorHak Hee Kim-
dc.contributor.googleauthorMi Hee Lee-
dc.contributor.googleauthorHyun Sook Baek-
dc.contributor.googleauthorKwon-Yong Lee-
dc.contributor.googleauthorSuong-Hyu Hyon-
dc.contributor.googleauthorJong-Chul Park-
dc.identifier.doi10.1007/s10529-005-3677-6-
dc.contributor.localIdA01095-
dc.contributor.localIdA01662-
dc.contributor.localIdA01843-
dc.contributor.localIdA02777-
dc.contributor.localIdA04275-
dc.relation.journalcodeJ00336-
dc.identifier.eissn1573-6776-
dc.identifier.urlhttp://link.springer.com/article/10.1007%2Fs10529-005-3677-6-
dc.contributor.alternativeNameKim, Hak Hee-
dc.contributor.alternativeNamePark, Jong Chul-
dc.contributor.alternativeNameBaek, Hyun Sook-
dc.contributor.alternativeNameLee, Mi Hee-
dc.contributor.alternativeNameHan, Dong Wook-
dc.citation.volume27-
dc.citation.number9-
dc.citation.startPage655-
dc.citation.endPage660-
dc.identifier.bibliographicCitationBiotechnology Letters, Vol.27(9) : 655-660, 2005-
dc.date.modified2017-05-04-
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers

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