0 454

Cited 10 times in

Pulmonary immunity and durable protection induced by the ID93/GLA-SE vaccine candidate against the hyper-virulent Korean Beijing Mycobacterium tuberculosis strain K

Authors
 Seung Bin Cha  ;  Woo Sik Kim  ;  Jong-Seok Kim  ;  Hongmin Kim  ;  Kee Woong Kwon  ;  Seung Jung Han  ;  Sang-Nae Cho  ;  Rhea N. Coler  ;  Steven G. Reed  ;  Sung Jae Shin 
Citation
 VACCINE, Vol.34(19) : 2179-2187, 2016 
Journal Title
 VACCINE 
ISSN
 0264-410X 
Issue Date
2016
MeSH
Animals ; Bacterial Load ; CD4-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/immunology ; Female ; Immunologic Memory ; Interferon-gamma/immunology ; Interleukin-2/immunology ; Lung/immunology* ; Lung/microbiology ; Lung/pathology ; Mice, Inbred C57BL ; Mycobacterium tuberculosis/classification ; Spleen/immunology ; Spleen/microbiology ; Tuberculosis/immunology ; Tuberculosis/prevention & control* ; Tuberculosis Vaccines/immunology* ; Tumor Necrosis Factor-alpha/immunology
Keywords
Beijing family ; GLA-SE ; ID93 ; K strain ; Tuberculosis
Abstract
The majority of tuberculosis (TB) vaccine candidates advanced to clinical trials have been evaluated preclinically using laboratory-adapted strains. However, it has been proposed that challenge with clinical isolates in preclinical vaccine testing could provide further and more practical validation. Here, we tested the ID93/GLA-SE TB vaccine candidate against the clinical Mycobacterium tuberculosis (Mtb) strain K (Mtb K) belonging to the Beijing family, the most prevalent Mtb strain in South Korea. Mice immunized with ID93/GLA-SE exhibited a significant reduction in bacteria and reduced lung inflammation against Mtb K when compared to non-immunized controls. In addition, we analyzed the immune responses in the lungs of ID93/GLA-SE-immunized mice, and showed that ID93/GLA-SE was able to elicit sustained Th1-biased immune responses including antigen-specific multifunctional CD4(+) T cell co-producing IFN-γ, TNF-α, and IL-2 as well as a high magnitude of IFN-γ response for up to 10 weeks post-challenge. Notably, further investigation of T cell subsets in the lung following challenge showed remarkable generation of CD8(+) central memory T cells by ID93/GLA-SE-immunization. Our findings showed that ID93/GLA-SE vaccine confers a high level of robust protection against the hypervirulent Mtb Beijing infection which was characterized by pulmonary Th1-polarized T-cell immune responses. These findings may also provide relevant information for potential utility of this vaccine candidate in East-Asian countries where the Beijing genotype is highly prevalent.
Full Text
http://www.sciencedirect.com/science/article/pii/S0264410X16003406
DOI
10.1016/j.vaccine.2016.03.029
Appears in Collections:
5. Research Institutes (연구소) > Institute for Immunology and Immunological Disease (면역질환연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jong Seok(김종석)
Shin, Sung Jae(신성재) ORCID logo https://orcid.org/0000-0003-0854-4582
Cho, Sang Nae(조상래)
Cha, Seung Bin(차승빈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146835
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links