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Femto-molar detection of cancer marker-protein based on immuno-nanoplasmonics at single-nanoparticle scale

Authors
 Yoochan Hong  ;  Eugene Lee  ;  Minhee Ku  ;  Jin-Suck Suh  ;  Dae Sung Yoon  ;  Jaemoon Yang 
Citation
 NANOTECHNOLOGY, Vol.27(18) : 185103, 2016 
Journal Title
 NANOTECHNOLOGY 
ISSN
 0957-4484 
Issue Date
2016
MeSH
Aptamers, Peptide/chemistry ; Aptamers, Peptide/metabolism* ; Biomarkers, Tumor/metabolism* ; Biosensing Techniques ; Cell Line, Tumor ; Epithelial Cell Adhesion Molecule/metabolism* ; Humans ; MCF-7 Cells ; Metal Nanoparticles/chemistry ; Neoplasms/metabolism* ; Silver/chemistry* ; Single-Cell Analysis ; Surface Plasmon Resonance/methods
Keywords
localized surface plasmon resonance ; silver nanomarble ; aptamer ; cancer ; single nanoparticle spectroscopy
Abstract
We describe an in vitro biomarker sensor based on immuno-silver nanomarbles (iSNMs) and the nanoscattering spectrum imaging analysis system using localized surface plasmon resonance (LSPR). In particular, highly monodisperse SNMs with large figures of merit are prepared, and the sensing substrates are also fabricated using the nanoparticle adsorption method. The high sensitivity of the LSPR sensor based on an SNM is confirmed using various solvents that have different refractive indexes. For the sensitive and specific detection of epithelial cell adhesion molecules (EpCAMs) expressed on cancer cells, the surface of the SNM is conjugated with an anti-EpCAM aptamer, and molecular sensing for the EpCAM expression level is carried out using whole cell lysates from various cancer cell lines. Collectively, we have developed a biomarker-detectable LSPR sensor based on iSNMs, which allows for the sensitive and effective detection of EpCAMs at both the single-cell and femto-molar level.
Full Text
http://iopscience.iop.org/article/10.1088/0957-4484/27/18/185103
DOI
10.1088/0957-4484/27/18/185103
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Ku, Min Hee(구민희) ORCID logo https://orcid.org/0000-0002-1674-1474
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146818
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