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Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells

Authors
 Chooryung J. Chung  ;  Euiseong Kim  ;  Minju Song  ;  Jeong-Won Park  ;  Su-Jung Shin 
Citation
 ODONTOLOGY, Vol.104 : 143-151, 2016 
Journal Title
 ODONTOLOGY 
ISSN
 1618-1247 
Issue Date
2016
MeSH
Angiogenesis Inducing Agents/pharmacology* ; Calcium/metabolism ; Calcium/pharmacology ; Cell Survival ; Cells, Cultured ; Dental Pulp/cytology* ; Fibroblast Growth Factor 2/metabolism ; Humans ; Materials Testing ; Ribonuclease, Pancreatic/metabolism ; Silicate Cement/pharmacology* ; Silicates/pharmacology ; Vascular Endothelial Growth Factor A/metabolism
Keywords
Calcium-silicate cement ; Fast setting ; Mineral trioxide aggregate ; Pozzolan cement ; Pulp cell
Abstract
Mineral trioxide aggregate (MTA) is considered a pulp-capping agent of choice, but has the drawback of a long setting time. This study aimed to assess two different types of calcium-silicate cements as pulp-capping agents, by investigating their in vitro cytotoxicity and angiogenic effects in human pulp cells. ProRoot MTA, Endocem Zr, and Retro MTA were prepared as set or freshly mixed pellets. Human pulp-derived cells were grown in direct contact with these three cements, Dycal, or no cement, for 7 days. Initial cell attachment, viability, calcium release, and the levels of vascular endothelial growth factor (VEGF), angiogenin, and basic fibroblast growth factor (FGF-2) were evaluated statistically using a linear mixed model (P < 0.05). The biocompatibility of Retro MTA was similar to those of the control and ProRoot MTA. Endocem Zr groups showed fewer and more rounded cells after a 3-day culture; however, the initial cytotoxicity appeared transient. All test materials showed significant increases in calcium concentration compared with the control group (P < 0.05). VEGF and angiogenin levels in ProRoot MTA and Retro MTA groups were significantly higher than those in the Endocem Zr group (P < 0.05). FGF-2 levels were not significantly different between groups (P > 0.05). We demonstrate that Retro MTA, which has a short setting time, has similar biocompatibility and angiogenic effects on human pulp cells, and can therefore potentially be as effective in pulp capping as ProRoot MTA. Endocem Zr showed intermittent cytotoxicity and elicited lower levels of VEGF and angiogenin expression.
Full Text
http://link.springer.com/article/10.1007/s10266-015-0194-5
DOI
10.1007/s10266-015-0194-5
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Orthodontics (교정과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eui Seong(김의성) ORCID logo https://orcid.org/0000-0003-2126-4761
Park, Jeong Won(박정원) ORCID logo https://orcid.org/0000-0002-2517-8150
Shin, Su Jung(신수정) ORCID logo https://orcid.org/0000-0001-5795-3919
Chung, Chooryung J.(정주령) ORCID logo https://orcid.org/0000-0001-9399-7193
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146698
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