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Therapeutic effects of late outgrowth endothelial progenitor cells or mesenchymal stem cells derived from human umbilical cord blood on infarct repair

 Sung-Whan Kim  ;  Hong Lian Jin  ;  Seok-Min Kang  ;  Sinyoung Kim  ;  Kyung-Jong Yoo  ;  Yangsoo Jang  ;  Hyun Ok Kim  ;  Young-sup Yoon 
 International Journal of Cardiology, Vol.203 : 498-507, 2016 
Journal Title
 International Journal of Cardiology 
Issue Date
Animals ; Cells, Cultured ; Disease Models, Animal ; Endothelial Progenitor Cells/transplantation* ; Human Umbilical Vein Endothelial Cells*/metabolism ; Humans ; Male ; Mesenchymal Stem Cell Transplantation*/methods ; Myocardial Infarction/therapy* ; Rats ; Rats, Nude ; Rats, Sprague-Dawley ; Wound Healing
Acute myocardial infarction ; Angiogenesis ; Late outgrowing endothelial progenitor cells ; Mesenchymal stem cells ; Umbilical cord blood
BACKGROUND: This study sought to systematically investigate the derivation of late outgrowth endothelial progenitor cells (late EPC) and mesenchymal stem cells (MSC) from umbilical cord blood (UCB) and to examine their therapeutic effects on myocardial infarction (MI). METHODS: The expression of angiogenic genes was determined by qRT-PCR. Myocardial infarction (MI) was induced in rats, and cells were directly transplanted into the border regions of ischemic heart tissue. RESULTS: Culture of UCB mononuclear cells yielded two distinct types of cells by morphology after 2 weeks in the same culture conditions. These cells were identified as late EPC and MSC, and each was intramyocardially injected into rat hearts after induction of MI. Echocardiography and histologic analyses demonstrated that both EPC and MSC improved cardiac function and enhanced vascularization, although fibrosis was reduced only in the EPC transplanted hearts. Different paracrine factors were enriched in EPC and MSC. However, once injected into the hearts, they induced similar types of paracrine factors in the heart. Transplanted EPC or MSC were mostly localized at the perivascular areas. This study demonstrated that EPC and MSC can be simultaneously derived from UCB under the same initial culture conditions, and that common paracrine factors are involved in the repair of MI. CONCLUSION: Late EPC and MSC are effective for infarct repair, apparently mediated through common humoral mechanisms.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
강석민(Kang, Seok Min) ORCID logo https://orcid.org/0000-0001-9856-9227
김신영(Kim, Sin Young) ORCID logo https://orcid.org/0000-0002-2609-8945
김현옥(Kim, Hyun Ok) ORCID logo https://orcid.org/0000-0002-4964-1963
유경종(Yoo, Kyung Jong) ORCID logo https://orcid.org/0000-0002-9858-140X
윤영섭(Yoon, Young Sup)
장양수(Jang, Yang Soo) ORCID logo https://orcid.org/0000-0002-2169-3112
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