0 596

Cited 10 times in

Bone Regenerative Efficacy of Limited-Dose Escherichia Coli-Derived rhBMP-2 With Biphasic Calcium Phosphate Carrier in Rabbit Calvarial Defect Model.

Authors
 Hoon You  ;  So-Ra Yoon  ;  Hyun-Chang Lim  ;  Jung-Seok Lee  ;  Ui-Won Jung  ;  Seong-Ho Choi 
Citation
 IMPLANT DENTISTRY, Vol.25(1) : 16-23, 2016 
Journal Title
IMPLANT DENTISTRY
ISSN
 1056-6163 
Issue Date
2016
MeSH
Animals ; Bone Morphogenetic Protein 2/therapeutic use* ; Bone Regeneration/drug effects* ; Escherichia coli/metabolism ; Hydroxyapatites/therapeutic use* ; Male ; Rabbits ; Recombinant Proteins/therapeutic use ; Skull/growth & development ; X-Ray Microtomography
Keywords
biphasic calcium phosphate ; bone morphogenetic protein 2 ; bone regeneration ; bone substitutes ; rabbit calvaria
Abstract
PURPOSE: The aim of this study was to characterize the healing in rabbit calvarial bone defects after delivery of limited-dose (1.5 μg) Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2), and evaluate biphasic calcium phosphate (BCP) as a carrier.
MATERIALS AND METHODS: Four 8-mm diameter circular calvarial bone defects were made in 16 rabbits and filled with one of the following: (1) nothing, (2) BCP alone, (3) ErhBMP-2-loaded absorbable collagen sponge (ACS), or (4) ErhBMP-2-loaded BCP. The animals were allowed to heal for either 2 or 8 weeks and were evaluated in clinical, microcomputed tomographic, histological, and histomorphometric analyses.
RESULTS: Microcomputed tomography revealed extensive new bone formation in both of the limited-dose ErhBMP-2-loaded groups. However, bony collapse of the upper defect borders was found in the ErhBMP-2-loaded ACS group. Histomorphometric examination revealed significantly greater new bone formation at 8 weeks than at 2 weeks in all 4 groups (P < 0.05). Both new bone formation and the size of the augmented area differed significantly between the ErhBMP-2-loaded BCP group (6.88 ± 0.74 and 19.62 ± 0.77) and the ErhBMP-2-loaded ACS group (3.04 ± 0.27 and 5.41 ± 0.43) at 8 weeks of healing.
CONCLUSION: ErhBMP-2 promotes bone regeneration in rabbit calvarial defects, even at a limited dose (1.5 μg). The results of this study suggest that BCP is the more efficient carrier for this protein than ACS.
Full Text
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00008505-201602000-00004&LSLINK=80&D=ovft
DOI
10.1097/ID.0000000000000364
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
You, Hoon(유훈)
Lee, Jung Seok(이중석) ORCID logo https://orcid.org/0000-0003-1276-5978
Jung, Ui Won(정의원) ORCID logo https://orcid.org/0000-0001-6371-4172
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/146342
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links