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Silver nanoparticles affect glucose metabolism in hepatoma cells through production of reactive oxygen species

Authors
 Mi Jin Lee  ;  Seung Jun Lee  ;  Su Jin Yun  ;  Ji-Young Jang  ;  Hangoo Kang  ;  Kyongmin Kim  ;  In-Hong Choi  ;  Sun Park 
Citation
 INTERNATIONAL JOURNAL OF NANOMEDICINE, Vol.11 : 55-68, 2016 
Journal Title
INTERNATIONAL JOURNAL OF NANOMEDICINE
ISSN
 1176-9114 
Issue Date
2016
MeSH
Antineoplastic Agents/chemistry ; Antineoplastic Agents/pharmacology* ; Carcinoma, Hepatocellular/drug therapy ; Carcinoma, Hepatocellular/metabolism ; Cell Line, Tumor ; Cell Survival/drug effects ; Glucose/metabolism* ; Hep G2 Cells/drug effects ; Hep G2 Cells/metabolism ; Humans ; Lactic Acid/metabolism ; Liver Neoplasms/drug therapy ; Liver Neoplasms/metabolism ; Metal Nanoparticles*/chemistry ; Particle Size ; Reactive Oxygen Species/metabolism* ; Silver/chemistry ; Silver/pharmacology*
Keywords
carbohydrate metabolism ; cytotoxicity ; lactic acid ; metal nanoparticles
Abstract
The silver nanoparticle (AgNP) is a candidate for anticancer therapy because of its effects on cell survival and signaling. Although numerous reports are available regarding their effect on cell death, the effect of AgNPs on metabolism is not well understood. In this study, we investigated the effect of AgNPs on glucose metabolism in hepatoma cell lines. Lactate release from both HepG2 and Huh7 cells was reduced with 5 nm AgNPs as early as 1 hour after treatment, when cell death did not occur. Treatment with 5 nm AgNPs decreased glucose consumption in HepG2 cells but not in Huh7 cells. Treatment with 5 nm AgNPs reduced nuclear factor erythroid 2-like 2 expression in both cell types without affecting its activation at the early time points after AgNPs' treatment. Increased reactive oxygen species (ROS) production was detected 1 hour after 5 nm AgNPs' treatment, and lactate release was restored in the presence of an ROS scavenger. Our results suggest that 5 nm AgNPs affect glucose metabolism by producing ROS.
Files in This Item:
T201600061.pdf Download
DOI
10.2147/IJN.S94907
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/145531
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