Cited 0 times in

Effects of MK-801 and Morphine on Spinal C-Fos Expression during the Development of Neuropathic Pain

 Youn Woo Lee  ;  Kyung Ah Park  ;  Won Taek Lee 
 YONSEI MEDICAL JOURNAL, Vol.43(3) : 370-376, 2002 
Journal Title
Issue Date
Allodynia ; central sensitization ; spinal c-fos ; MK-801 ; morphine
The purpose of this study was to investigate the expression of c-fos in the spinal cord during the development of allodynia, induced by peripheral nerve injury. Following tight ligation of the left L5 and L6 spinal nerves of Sprague-Dawley rat, the lumbar spinal cord was postfixed following perfusion. Frontal frozen sections of 40 µm were immunostained according to the peroxidase-antiperoxidase method. The allodynic threshold was checked with 8 calibrated von Frey filaments. MK-801 (0.3 mg/kg), morphine (3 mg/kg) and saline (as a placebo) were administered subcutaneously 30 min before, and 24 and 48 hrs after surgery. The tactile threshold decreased below 3 g since 2 days after surgery in the saline and morphine groups, but delayed a little in the MK-801 group. In the superficial layer the number of Fos-like immunoreactive neurones (Fos-LI) peaked at 2 hours and decreased thereafter, and reached normal levels 24 hrs following operation, for all groups. In the deep layer they were biphasic, - peaking at 2 and 24 hrs - in the saline group, but were suppressed in the morphine and MK-801 groups until 7 days following operation. The above discrepancy between the number of Fos-LI and the allodynic threshold showed that central sensitizations are not critically involved in the development of nerve injury induced tactile allodynia.
Files in This Item:
T200210912.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Park, Kyung Ah(박경아)
Lee, Won Taek(이원택) ORCID logo
Lee, Youn Woo(이윤우)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.