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Effects of MK-801 and Morphine on Spinal C-Fos Expression during the Development of Neuropathic Pain

 Youn Woo Lee  ;  Kyung Ah Park  ;  Won Taek Lee 
 Yonsei Medical Journal, Vol.43(3) : 370-376, 2002 
Journal Title
 Yonsei Medical Journal 
Issue Date
The purpose of this study was to investigate the expression of c-fos in the spinal cord during the development of allodynia, induced by peripheral nerve injury. Following tight ligation of the left L5 and L6 spinal nerves of Sprague-Dawley rat, the lumbar spinal cord was postfixed following perfusion. Frontal frozen sections of 40 µm were immunostained according to the peroxidase-antiperoxidase method. The allodynic threshold was checked with 8 calibrated von Frey filaments. MK-801 (0.3 mg/kg), morphine (3 mg/kg) and saline (as a placebo) were administered subcutaneously 30 min before, and 24 and 48 hrs after surgery. The tactile threshold decreased below 3 g since 2 days after surgery in the saline and morphine groups, but delayed a little in the MK-801 group. In the superficial layer the number of Fos-like immunoreactive neurones (Fos-LI) peaked at 2 hours and decreased thereafter, and reached normal levels 24 hrs following operation, for all groups. In the deep layer they were biphasic, - peaking at 2 and 24 hrs - in the saline group, but were suppressed in the morphine and MK-801 groups until 7 days following operation. The above discrepancy between the number of Fos-LI and the allodynic threshold showed that central sensitizations are not critically involved in the development of nerve injury induced tactile allodynia.
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1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
박경아(Park, Kyung Ah)
이원택(Lee, Won Taek) ORCID logo https://orcid.org/0000-0001-7348-9562
이윤우(Lee, Youn Woo)
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