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Expression of MUC1 on Corneal Endothelium of Human.

Authors
 Seung Eun Jung  ;  Kyoung Yul Seo  ;  Hyun Kim  ;  Hyung Lae Kim  ;  In Hyuk Chung  ;  Eung Kweon Kim 
Citation
 CORNEA, Vol.21(7) : 691-695, 2002 
Journal Title
 CORNEA 
ISSN
 0277-3740 
Issue Date
2002
MeSH
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Endothelium, Corneal/metabolism* ; Humans ; Immunoblotting ; Immunohistochemistry ; In Situ Hybridization ; Middle Aged ; Mucin-1/genetics ; Mucin-1/metabolism* ; RNA, Messenger/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Tissue Distribution
Keywords
Corneal endothelium ; Lycoprotein ; Mucin ; MUC1 ; Gene expression
Abstract
Purpose. To determine the expression of MUC1 in the human corneal endothelium. Methods. Reverse transcription-polymerase chain reaction (RT-PCR) for MUC1 was performed with total RNA from endothelial cells extracted from the human cornea. In situ hybridization with sense and antisense probes of human MUC1 was performed on the human corneal endothelium, immunoblot analysis using monoclonal antibody specific for human MUC1 (HMFG-1, or VU4H5) was performed on collected human corneal endothelial cells, and immunohistochemistry on the human cornea, using the same antibodies. Results. MUC1 mRNA expression was observed by RT-PCR in the human corneal endothelium, and the nucleotide sequence from the amplified band was matched with known human MUC1. In situ hybridization studies showed the localization of MUC1 mRNA in the human corneal endothelium, and immunoblot assay demonstrated the presence of MUC1 protein (MW > 200 kd). In addition, MUC1 protein was observed on the apical surface of cells and at the superficial layer of the cytoplasm in immunohistochemical studies. Conclusions. Human corneal endothelial cells produce MUC1, which is known to have protective and lubricative roles.
Full Text
http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00003226-200210000-00011&LSLINK=80&D=ovft
DOI
10.1097/01.ICO.0000023393.68720.EF
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eung Kweon(김응권) ORCID logo https://orcid.org/0000-0002-1453-8042
Seo, Kyuong Yul(서경률) ORCID logo https://orcid.org/0000-0002-9855-1980
Chung, In Hyuk(정인혁)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143931
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