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Antibacterial Effect of Antibiotic Solution on Cellular Viability in Canine Veins

Authors
 Jong-Chul Park  ;  Hak-Joon Sung  ;  Dong Hee Lee  ;  Young Hwan Park  ;  Bum Koo Cho  ;  Hwal Suh 
Citation
 ARTIFICIAL ORGANS, Vol.25(6) : 490-494, 2001 
Journal Title
 ARTIFICIAL ORGANS 
ISSN
 0160-564X 
Issue Date
2001
MeSH
Animals ; Anti-Bacterial Agents/pharmacology* ; Bacterial Infections/prevention & control* ; Cefoxitin/pharmacology ; Dogs ; Endothelium, Vascular/cytology ; Endothelium, Vascular/drug effects ; Lincomycin/pharmacology ; Polymyxin B/pharmacology ; Solutions ; Statistics, Nonparametric ; Vancomycin/pharmacology ; Veins/drug effects* ; Veins/transplantation
Keywords
Antibiotic solution ; Sterilization effect ; Cellular viability ; Canine veins
Abstract
Pretreatment of tissue by using antibiotics is a critical step to prevent microbial contamination before venous transplantation. In this study, the optimal time and temperature of antibiotic solution treatment for maintaining cellular viability with antibacterial effect were investigated. The antibiotic-nutrient solutions were composed of cefoxitin, lincomycin, vancomycin, and polymyxin B in RPMI-1640 medium. After various antibiotic solution treatment times (4, 8, and 12 h) and temperatures (4, 25, and 37°C), the viabilities of cells dissociated from veins (jugular vein, femoral vein, superior vena cava, and inferior vena cava) were determined. Double staining by Griffonia simplicifolia agglutins–fluorescein isothiocyanate (GS1-FITC) and propidium iodide was used. To measure the antibacterial effect of the antibiotic solution, canine veins were artificially infected by 3 kinds of bacteria (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae) and were treated by antibiotic solutions as viability test conditions. After the treatment with the antibiotic solution, the tissue was minced, and the homogenized tissue fraction was cultured on standard method agar. The colony that seemed to be resistant to the antibiotic solution was counted. At 37 and 25°C, the viability of whole cells decreased significantly Asymptotic Significance 2-tailed (Asymp.Sig 2-tailed) < 0.05 after 4 h of antibiotic solution treatment, whereas at 4°C it began to reduce significantly after 8 h of treatment. By antibiotic solution treatment at all 3 temperatures for 4 h, no significant difference in viability of the endothelial cells and whole cells was observed. To maintain the donor vein's cellular viability until transplantation, antibiotic solution treatment for 4 h at 4°C is assumed to be appropriate.
Full Text
http://onlinelibrary.wiley.com/doi/10.1046/j.1525-1594.2001.06706-2.x/abstract
DOI
10.1046/j.1525-1594.2001.06706-2.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Hwan(박영환)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Suh, Hwal(서활)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143174
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