0 605

Cited 3 times in

Antibacterial Effect of Antibiotic Solution on Cellular Viability in Canine Veins

DC Field Value Language
dc.contributor.author박영환-
dc.contributor.author박종철-
dc.contributor.author서활-
dc.date.accessioned2016-02-19T11:27:09Z-
dc.date.available2016-02-19T11:27:09Z-
dc.date.issued2001-
dc.identifier.issn0160-564X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/143174-
dc.description.abstractPretreatment of tissue by using antibiotics is a critical step to prevent microbial contamination before venous transplantation. In this study, the optimal time and temperature of antibiotic solution treatment for maintaining cellular viability with antibacterial effect were investigated. The antibiotic-nutrient solutions were composed of cefoxitin, lincomycin, vancomycin, and polymyxin B in RPMI-1640 medium. After various antibiotic solution treatment times (4, 8, and 12 h) and temperatures (4, 25, and 37°C), the viabilities of cells dissociated from veins (jugular vein, femoral vein, superior vena cava, and inferior vena cava) were determined. Double staining by Griffonia simplicifolia agglutins–fluorescein isothiocyanate (GS1-FITC) and propidium iodide was used. To measure the antibacterial effect of the antibiotic solution, canine veins were artificially infected by 3 kinds of bacteria (Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae) and were treated by antibiotic solutions as viability test conditions. After the treatment with the antibiotic solution, the tissue was minced, and the homogenized tissue fraction was cultured on standard method agar. The colony that seemed to be resistant to the antibiotic solution was counted. At 37 and 25°C, the viability of whole cells decreased significantly Asymptotic Significance 2-tailed (Asymp.Sig 2-tailed) < 0.05 after 4 h of antibiotic solution treatment, whereas at 4°C it began to reduce significantly after 8 h of treatment. By antibiotic solution treatment at all 3 temperatures for 4 h, no significant difference in viability of the endothelial cells and whole cells was observed. To maintain the donor vein's cellular viability until transplantation, antibiotic solution treatment for 4 h at 4°C is assumed to be appropriate.-
dc.description.statementOfResponsibilityopen-
dc.format.extent490~494-
dc.relation.isPartOfARTIFICIAL ORGANS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHAnti-Bacterial Agents/pharmacology*-
dc.subject.MESHBacterial Infections/prevention & control*-
dc.subject.MESHCefoxitin/pharmacology-
dc.subject.MESHDogs-
dc.subject.MESHEndothelium, Vascular/cytology-
dc.subject.MESHEndothelium, Vascular/drug effects-
dc.subject.MESHLincomycin/pharmacology-
dc.subject.MESHPolymyxin B/pharmacology-
dc.subject.MESHSolutions-
dc.subject.MESHStatistics, Nonparametric-
dc.subject.MESHVancomycin/pharmacology-
dc.subject.MESHVeins/drug effects*-
dc.subject.MESHVeins/transplantation-
dc.titleAntibacterial Effect of Antibiotic Solution on Cellular Viability in Canine Veins-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Medical Engineering (의학공학)-
dc.contributor.googleauthorJong-Chul Park-
dc.contributor.googleauthorHak-Joon Sung-
dc.contributor.googleauthorDong Hee Lee-
dc.contributor.googleauthorYoung Hwan Park-
dc.contributor.googleauthorBum Koo Cho-
dc.contributor.googleauthorHwal Suh-
dc.identifier.doi10.1046/j.1525-1594.2001.06706-2.x-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01574-
dc.contributor.localIdA01662-
dc.contributor.localIdA01924-
dc.relation.journalcodeJ00246-
dc.identifier.eissn1525-1594-
dc.identifier.pmid11453881-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1046/j.1525-1594.2001.06706-2.x/abstract-
dc.subject.keywordAntibiotic solution-
dc.subject.keywordSterilization effect-
dc.subject.keywordCellular viability-
dc.subject.keywordCanine veins-
dc.contributor.alternativeNamePark, Young Hwan-
dc.contributor.alternativeNamePark, Jong Chul-
dc.contributor.alternativeNameSuh, Hwal-
dc.contributor.affiliatedAuthorPark, Young Hwan-
dc.contributor.affiliatedAuthorPark, Jong Chul-
dc.contributor.affiliatedAuthorSuh, Hwal-
dc.rights.accessRightsnot free-
dc.citation.volume25-
dc.citation.number6-
dc.citation.startPage490-
dc.citation.endPage494-
dc.identifier.bibliographicCitationARTIFICIAL ORGANS, Vol.25(6) : 490-494, 2001-
dc.identifier.rimsid39111-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.