0 655

Cited 31 times in

Activation of Matrix Metalloproteinase-2 by Novel Oral Spirochetal Species, Treponema lecithinolyticum

Authors
 Bong-Kyu Choi  ;  Jung-Hag Jung  ;  Hye-Yuhn Suh  ;  Yun-Jung Yoo  ;  Kyoo-Sung Cho  ;  Jung-Kiu Chai  ;  Chong-Kwan Kim 
Citation
 JOURNAL OF PERIODONTOLOGY, Vol.72(11) : 1594-1600, 2001 
Journal Title
JOURNAL OF PERIODONTOLOGY
ISSN
 0022-3492 
Issue Date
2001
MeSH
Animals ; Bacterial Outer Membrane Proteins/metabolism ; Cattle ; Cells, Cultured ; Collagen Type IV/metabolism ; Coloring Agents ; Electrophoresis, Polyacrylamide Gel ; Enzyme Activation ; Enzyme-Linked Immunosorbent Assay ; Extracellular Matrix/metabolism ; Fibroblasts/cytology ; Fibroblasts/enzymology ; Fibroblasts/microbiology ; Gingiva/cytology ; Gingiva/enzymology ; Gingiva/microbiology ; Humans ; Immunoblotting ; Matrix Metalloproteinase 2/metabolism* ; Periodontal Ligament/cytology ; Periodontal Ligament/enzymology ; Periodontal Ligament/microbiology ; Periodontitis/microbiology ; Tetrazolium Salts ; Thiazoles ; Time Factors ; Treponema/classification ; Treponema/enzymology*
Keywords
Metalloproteinases ; matrix ; periodontitis/complications ; Treponema lecithinolyticum
Abstract
BACKGROUND:
Periodontal tissue destruction is a characteristic of periodontitis. This can be caused by either bacterial enzymes or host cell-derived matrix metalloproteinases (MMPs). In order to elucidate the etiologic role of oral spirochetes, we investigated the effects of Treponema lecithinolyticum, a novel saccharolytic species, on MMP-2 activation.
METHODS:
Gingival fibroblasts (GFs) and periodontal ligament (PDL) cells obtained from healthy human subjects were cultured to confluence in alpha-minimal essential medium (alpha-MEM) supplemented with 10% fetal bovine serum. After serum starvation for a day, the cultures were treated with whole cell sonicates, heat-denatured whole cell sonicates, outer membrane fraction (OMF) or formaldehyde-fixed cells of T. lecithinolyticum. Culture supernatants were collected after incubation for 24 to 48 hours and analyzed for MMP-2 activation by gelatin zymography. Collagenolytic activity was quantitatively measured using human [3H] type IV collagen as a substrate.
RESULTS:
Treatment of GFs and PDL cells with whole cell sonicates, formaldehyde-fixed whole cells, or the OMF of T. lecithinolyticum resulted in the production of MMP-2 partly in the fully active form with a molecular mass of 62 kDa, whereas non-treated control cultures and cultures treated with a heat-denatured fraction did not show the active form. Cultures exposed to T. lecithinolyticum had higher collagenolytic activity than non-treated cultures.
CONCLUSIONS:
Our results demonstrate that T. lecithinolyticum, possibly mediated by proteinaceous cell surface-associated components, may participate in extracellular matrix degradation by activation of MMP-2 during periodontal inflammation.
Full Text
http://www.joponline.org/doi/abs/10.1902/jop.2001.72.11.1594
DOI
10.1902/jop.2001.72.11.1594
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chong Kwan(김종관)
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
Cho, Kyoo Sung(조규성) ORCID logo https://orcid.org/0000-0002-6777-5287
Chai, Jung Kyu(채중규)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143142
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links