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Identification of oral spirochetes at the species-level and their association with other bacteria in endodontic infections.

Authors
 Il-Young Jung  ;  Bong-kyu Choi  ;  Ki-Yeon Kum  ;  Yun-Jung Yoo  ;  Tai-Cheol Yoon  ;  Seung-Jong Lee  ;  Chan-Young Lee 
Citation
 ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, Vol.92(3) : 329-334, 2001 
Journal Title
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS
ISSN
 1079-2104 
Issue Date
2001
MeSH
Adolescent ; Adult ; Aged ; Bacteroides/classification* ; Bacteroides/genetics ; Child ; Confidence Intervals ; DNA Probes ; DNA, Bacterial/genetics ; Dental Pulp Necrosis/microbiology* ; Humans ; Immunoblotting ; Middle Aged ; Nucleic Acid Hybridization ; Odds Ratio ; Periapical Periodontitis/microbiology ; Polymerase Chain Reaction ; Porphyromonas gingivalis/classification* ; Porphyromonas gingivalis/genetics ; RNA, Ribosomal, 16S/genetics ; Spirochaetales/classification* ; Spirochaetales/genetics ; Treponema/classification ; Treponema/genetics
Abstract
OBJECTIVES:
Recent molecular approaches have revealed that fastidious organisms such as Bacteroides forsythus and oral treponemes were frequently found in root canals with apical periodontitis. The purpose of this study was to identify the isolates of oral spirochetes at the species level in endodontic infections and to determine their association with B forsythus and Porphyromonas gingivalis.
STUDY DESIGN:
Seventy-nine teeth with apical periodontitis were selected for this study. After sampling from the root canals aseptically, polymerase chain reaction amplification for the 16S rRNA gene was performed with eubacterial universal primers. Subsequently, dot-blot hybridization was performed with 8 species-specific oligonucleotide probes. The microbial associations were analyzed by using the odds ratio.
RESULTS:
The most frequently found species was P gingivalis (27.4%), followed by Treponema maltophilum (26%), B forsythus (16.4%), and Treponema socranskii (2.7%). Other treponemes, including Treponema denticola, were not detected in our samples. Significant microbial associations were identified between T maltophilum, B forsythus, and P gingivalis by performing analysis with the odds ratio.
CONCLUSION:
Our results indicate that T maltophilum should be included in etiologic studies of endodontic diseases.
Full Text
http://www.sciencedirect.com/science/article/pii/S1079210401788968
DOI
10.1067/moe.2001.117263
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
Lee, Seung Jong(이승종)
Jung, Il Young(정일영) ORCID logo https://orcid.org/0000-0001-8972-2664
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/143112
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