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Helicobacter pylori-induced expression of interleukin-8 and cyclooxygenase-2 in AGS gastric epithelial cells: mediation by nuclear factor-kappaB

Authors
 H. Kim  ;  J. W. Lim  ;  K. H. Kim 
Citation
 SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, Vol.36(7) : 706-716, 2001 
Journal Title
 SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY 
ISSN
 0036-5521 
Issue Date
2001
MeSH
Adenocarcinoma/immunology* ; Adenocarcinoma/microbiology* ; Adenocarcinoma/pathology ; Antioxidants/pharmacology ; Cyclooxygenase 2 ; Drug Evaluation, Preclinical ; Epithelium ; Gastric Mucosa/cytology* ; Gastric Mucosa/immunology* ; Gastritis/immunology* ; Gastritis/microbiology* ; Gastritis/pathology ; Gene Expression Regulation, Neoplastic/drug effects* ; Gene Expression Regulation, Neoplastic/immunology* ; Glutathione/pharmacology ; Helicobacter Infections/complications* ; Helicobacter Infections/immunology* ; Helicobacter Infections/pathology ; Helicobacter pylori* ; Humans ; Immunity, Mucosal ; Interleukin-8/analysis ; Interleukin-8/physiology* ; Isoenzymes/analysis ; Isoenzymes/drug effects* ; Isoenzymes/physiology* ; Membrane Proteins ; NF-kappa B/antagonists & inhibitors ; NF-kappa B/drug effects* ; NF-kappa B/physiology* ; Oligonucleotides, Antisense/pharmacology ; Prostaglandin-Endoperoxide Synthases/analysis ; Prostaglandin-Endoperoxide Synthases/drug effects* ; Prostaglandin-Endoperoxide Synthases/physiology* ; Pyrrolidines/pharmacology ; Stomach Neoplasms/immunology* ; Stomach Neoplasms/microbiology* ; Stomach Neoplasms/pathology ; Thiocarbamates/pharmacology ; Time Factors ; Transcription, Genetic/drug effects ; Transcription, Genetic/physiology ; Tumor Cells, Cultured
Keywords
Cyclooxygenase-2 ; Gastric Epithelial Cells ; Helicobacter Pylori Interleukin ; Nf-KAPPA-B
Abstract
BACKGROUND: Helicobacter pylori infection might activate nuclear factor-kappaB (NF-kappaB), a transcriptional regulator of inducible expression of inflammatory genes, interleukin-8 (IL-8) and cyclooxygenase-2 (COX-2). We studied the role of NF-kappaB on expression of IL-8 and COX-2 in H. pylori-stimulated AGS gastric epithelial cells by using antisense oligonucleotide (AS ODN) for NF-kappaB subunit p50 and an antioxidant, glutathione (GSH) as well as a NF-kappaB inhibitor, pyrrolidine dithiocarbamate (PDTC). METHODS: AGS cells were treated with p50 AS ODN, GSH or PDTC in the presence of H. pylori. mRNA expression and protein levels for IL-8 and COX-2 were determined by Northern blot analysis and Western blot analysis. Levels of IL-8, 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) and thromboxane B2 (TXB2) were measured in the medium by enzyme-linked immunosorbent assay. NF-kappaB activation was examined by electrophoretic mobility shift assay. RESULTS: H. pylori induced a time-dependent expression of mRNA and protein for IL-8 and COX-2 via activation of NF-kappaB and increased the levels of IL-8, 6-keto-PGF1alpha and TXB2, which were inhibited by GSH and PDTC. H. pylori-induced expression of IL-8 and COX-2 was blocked in AGS cells transfected with p50 AS ODN. CONCLUSION: NF-kappaB may play a novel role in expression of IL-8 and COX-2 in H. pylori-induced gastric inflammation.
Full Text
http://informahealthcare.com/doi/abs/10.1080/00365520119046
DOI
10.1080/00365520119046
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/141959
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