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Helicobacter pylori-induced expression of interleukin-8 and cyclooxygenase-2 in AGS gastric epithelial cells: mediation by nuclear factor-kappaB
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김경환 | - |
dc.date.accessioned | 2016-02-19T10:54:16Z | - |
dc.date.available | 2016-02-19T10:54:16Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0036-5521 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/141959 | - |
dc.description.abstract | BACKGROUND: Helicobacter pylori infection might activate nuclear factor-kappaB (NF-kappaB), a transcriptional regulator of inducible expression of inflammatory genes, interleukin-8 (IL-8) and cyclooxygenase-2 (COX-2). We studied the role of NF-kappaB on expression of IL-8 and COX-2 in H. pylori-stimulated AGS gastric epithelial cells by using antisense oligonucleotide (AS ODN) for NF-kappaB subunit p50 and an antioxidant, glutathione (GSH) as well as a NF-kappaB inhibitor, pyrrolidine dithiocarbamate (PDTC). METHODS: AGS cells were treated with p50 AS ODN, GSH or PDTC in the presence of H. pylori. mRNA expression and protein levels for IL-8 and COX-2 were determined by Northern blot analysis and Western blot analysis. Levels of IL-8, 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) and thromboxane B2 (TXB2) were measured in the medium by enzyme-linked immunosorbent assay. NF-kappaB activation was examined by electrophoretic mobility shift assay. RESULTS: H. pylori induced a time-dependent expression of mRNA and protein for IL-8 and COX-2 via activation of NF-kappaB and increased the levels of IL-8, 6-keto-PGF1alpha and TXB2, which were inhibited by GSH and PDTC. H. pylori-induced expression of IL-8 and COX-2 was blocked in AGS cells transfected with p50 AS ODN. CONCLUSION: NF-kappaB may play a novel role in expression of IL-8 and COX-2 in H. pylori-induced gastric inflammation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 706~716 | - |
dc.relation.isPartOf | SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenocarcinoma/immunology* | - |
dc.subject.MESH | Adenocarcinoma/microbiology* | - |
dc.subject.MESH | Adenocarcinoma/pathology | - |
dc.subject.MESH | Antioxidants/pharmacology | - |
dc.subject.MESH | Cyclooxygenase 2 | - |
dc.subject.MESH | Drug Evaluation, Preclinical | - |
dc.subject.MESH | Epithelium | - |
dc.subject.MESH | Gastric Mucosa/cytology* | - |
dc.subject.MESH | Gastric Mucosa/immunology* | - |
dc.subject.MESH | Gastritis/immunology* | - |
dc.subject.MESH | Gastritis/microbiology* | - |
dc.subject.MESH | Gastritis/pathology | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic/drug effects* | - |
dc.subject.MESH | Gene Expression Regulation, Neoplastic/immunology* | - |
dc.subject.MESH | Glutathione/pharmacology | - |
dc.subject.MESH | Helicobacter Infections/complications* | - |
dc.subject.MESH | Helicobacter Infections/immunology* | - |
dc.subject.MESH | Helicobacter Infections/pathology | - |
dc.subject.MESH | Helicobacter pylori* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunity, Mucosal | - |
dc.subject.MESH | Interleukin-8/analysis | - |
dc.subject.MESH | Interleukin-8/physiology* | - |
dc.subject.MESH | Isoenzymes/analysis | - |
dc.subject.MESH | Isoenzymes/drug effects* | - |
dc.subject.MESH | Isoenzymes/physiology* | - |
dc.subject.MESH | Membrane Proteins | - |
dc.subject.MESH | NF-kappa B/antagonists & inhibitors | - |
dc.subject.MESH | NF-kappa B/drug effects* | - |
dc.subject.MESH | NF-kappa B/physiology* | - |
dc.subject.MESH | Oligonucleotides, Antisense/pharmacology | - |
dc.subject.MESH | Prostaglandin-Endoperoxide Synthases/analysis | - |
dc.subject.MESH | Prostaglandin-Endoperoxide Synthases/drug effects* | - |
dc.subject.MESH | Prostaglandin-Endoperoxide Synthases/physiology* | - |
dc.subject.MESH | Pyrrolidines/pharmacology | - |
dc.subject.MESH | Stomach Neoplasms/immunology* | - |
dc.subject.MESH | Stomach Neoplasms/microbiology* | - |
dc.subject.MESH | Stomach Neoplasms/pathology | - |
dc.subject.MESH | Thiocarbamates/pharmacology | - |
dc.subject.MESH | Time Factors | - |
dc.subject.MESH | Transcription, Genetic/drug effects | - |
dc.subject.MESH | Transcription, Genetic/physiology | - |
dc.subject.MESH | Tumor Cells, Cultured | - |
dc.title | Helicobacter pylori-induced expression of interleukin-8 and cyclooxygenase-2 in AGS gastric epithelial cells: mediation by nuclear factor-kappaB | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | H. Kim | - |
dc.contributor.googleauthor | J. W. Lim | - |
dc.contributor.googleauthor | K. H. Kim | - |
dc.identifier.doi | 10.1080/00365520119046 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00311 | - |
dc.relation.journalcode | J02632 | - |
dc.identifier.eissn | 1502-7708 | - |
dc.identifier.pmid | 11444469 | - |
dc.identifier.url | http://informahealthcare.com/doi/abs/10.1080/00365520119046 | - |
dc.subject.keyword | Cyclooxygenase-2 | - |
dc.subject.keyword | Gastric Epithelial Cells | - |
dc.subject.keyword | Helicobacter Pylori Interleukin | - |
dc.subject.keyword | Nf-KAPPA-B | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 36 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 706 | - |
dc.citation.endPage | 716 | - |
dc.identifier.bibliographicCitation | SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, Vol.36(7) : 706-716, 2001 | - |
dc.identifier.rimsid | 35881 | - |
dc.type.rims | ART | - |
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